Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform
Targeted delivery systems combined with the stimuli-responsive release of drug molecules hold noteworthy promise for precision medicine, enabling treatments with enhanced effectiveness and reduced adverse effects. An ideal drug delivery platform with versatile targeting moieties, the capability of c...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/181045 |
| _version_ | 1826126323198722048 |
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| author | Wang, Xian Seah, Hui Ling Zhang, Xiao-Lin Zhuang, Zeyan Liu, Xue-Wei |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Wang, Xian Seah, Hui Ling Zhang, Xiao-Lin Zhuang, Zeyan Liu, Xue-Wei |
| author_sort | Wang, Xian |
| collection | NTU |
| description | Targeted delivery systems combined with the stimuli-responsive release of drug molecules hold noteworthy promise for precision medicine, enabling treatments with enhanced effectiveness and reduced adverse effects. An ideal drug delivery platform with versatile targeting moieties, the capability of combinational payloads, and simple preparation is highly desirable. Herein, we developed pH-sensitive fluorescent self-assembled complexes (SACs) of a galactose-functionalized G-quadruplex (G4) and a coumarin carboxamidine derivative as a targeted delivery platform through the nanoprecipitation method. These SACs selectively targeted hepatocellular carcinoma (HepG2) cells in fluorescence imaging after a short incubation and exerted specific anticancer effects in an appropriate dose range. Co-delivery of 1 μM prodrug floxuridine oligomers and 16 μg/mL SACs (minimal hemolytic effect) significantly reduced the cytotoxicity of the nucleoside anticancer drug on normal cells (NIH/3T3), kept up to 70% alive after 72-h incubation, and improved anticancer efficacy compared to SACs alone. This strategy can be extended to ratiometric multidrug delivery through self-assembly for targeted combinational therapy. |
| first_indexed | 2025-03-09T14:16:12Z |
| format | Journal Article |
| id | ntu-10356/181045 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-03-09T14:16:12Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | ntu-10356/1810452024-11-12T05:46:21Z Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform Wang, Xian Seah, Hui Ling Zhang, Xiao-Lin Zhuang, Zeyan Liu, Xue-Wei School of Chemistry, Chemical Engineering and Biotechnology School of Physical and Mathematical Sciences Engineering Fluorescence imaging Fluorescent self-assembled complex Targeted delivery systems combined with the stimuli-responsive release of drug molecules hold noteworthy promise for precision medicine, enabling treatments with enhanced effectiveness and reduced adverse effects. An ideal drug delivery platform with versatile targeting moieties, the capability of combinational payloads, and simple preparation is highly desirable. Herein, we developed pH-sensitive fluorescent self-assembled complexes (SACs) of a galactose-functionalized G-quadruplex (G4) and a coumarin carboxamidine derivative as a targeted delivery platform through the nanoprecipitation method. These SACs selectively targeted hepatocellular carcinoma (HepG2) cells in fluorescence imaging after a short incubation and exerted specific anticancer effects in an appropriate dose range. Co-delivery of 1 μM prodrug floxuridine oligomers and 16 μg/mL SACs (minimal hemolytic effect) significantly reduced the cytotoxicity of the nucleoside anticancer drug on normal cells (NIH/3T3), kept up to 70% alive after 72-h incubation, and improved anticancer efficacy compared to SACs alone. This strategy can be extended to ratiometric multidrug delivery through self-assembly for targeted combinational therapy. Ministry of Education (MOE) We thank the Ministry of Education (MOE-T2EP30120-0007 and MOE2018-T2-2-128) from Singapore for their generous financial support. 2024-11-12T05:46:21Z 2024-11-12T05:46:21Z 2024 Journal Article Wang, X., Seah, H. L., Zhang, X., Zhuang, Z. & Liu, X. (2024). Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform. ACS Applied Materials & Interfaces, 16(38), 50229-50237. https://dx.doi.org/10.1021/acsami.4c08079 1944-8244 https://hdl.handle.net/10356/181045 10.1021/acsami.4c08079 39264898 2-s2.0-85203839255 38 16 50229 50237 en MOE-T2EP30120-0007 MOE2018-T2-2-128 ACS Applied Materials & Interfaces © 2024 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering Fluorescence imaging Fluorescent self-assembled complex Wang, Xian Seah, Hui Ling Zhang, Xiao-Lin Zhuang, Zeyan Liu, Xue-Wei Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform |
| title | Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform |
| title_full | Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform |
| title_fullStr | Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform |
| title_full_unstemmed | Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform |
| title_short | Fluorescent self-assembled complexes based on glyco-functionalized G-quadruplexes as a targeted delivery platform |
| title_sort | fluorescent self assembled complexes based on glyco functionalized g quadruplexes as a targeted delivery platform |
| topic | Engineering Fluorescence imaging Fluorescent self-assembled complex |
| url | https://hdl.handle.net/10356/181045 |
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