Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release
Wounds pose a risk to the skin, our body’s primary defence against infections. The rise of antibiotic resistance has prompted the development of novel therapies. RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide (H2O2), a reactive oxygen species, directly to the w...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2025
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| Online Access: | https://hdl.handle.net/10356/182455 |
| _version_ | 1826115024929685504 |
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| author | Mieles, Joel Yupanqui Vyas, Cian Daskalakis, Evangelos Hassan, Mohamed Birkett, James Omar, Abdalla M. Humphreys, Gavin Diver, Carl Bartolo, Paulo |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Mieles, Joel Yupanqui Vyas, Cian Daskalakis, Evangelos Hassan, Mohamed Birkett, James Omar, Abdalla M. Humphreys, Gavin Diver, Carl Bartolo, Paulo |
| author_sort | Mieles, Joel Yupanqui |
| collection | NTU |
| description | Wounds pose a risk to the skin, our body’s primary defence against infections. The rise of antibiotic resistance has prompted the development of novel therapies. RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide (H2O2), a reactive oxygen species, directly to the wound bed. In this study, electrospinning was used to incorporate RO-101® into a polyvinyl alcohol (PVA) sub-micron fibrous mesh that can act as a delivery agent, achieve a sustained release profile, and provide a barrier against infection. Adequate incorporation of this gel into sub-micron fibres was confirmed via nuclear magnetic resonance spectroscopy. Furthermore, scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200–500 nm range. PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(g·mL) (1 mM = 1 mmol/L) after 24 h, and the role of sterilisation on H2O2 release was evaluated. PVA/RO-101 meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) bacteria, achieving viable count reductions of up to 1 log unit CFU/mm2 (CFU: colony-forming units). Moreover, these meshes were capable of disrupting biofilm formation, even against multidrug-resistant organisms such as methicillin-resistant S. aureus (MRSA). Furthermore, increasing the RO-101® concentration resulted in higher H2O2 production and an enhanced antimicrobial effect, while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101® concentrations. This study therefore demonstrates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings. |
| first_indexed | 2025-03-09T11:16:38Z |
| format | Journal Article |
| id | ntu-10356/182455 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-03-09T11:16:38Z |
| publishDate | 2025 |
| record_format | dspace |
| spelling | ntu-10356/1824552025-02-08T16:49:35Z Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release Mieles, Joel Yupanqui Vyas, Cian Daskalakis, Evangelos Hassan, Mohamed Birkett, James Omar, Abdalla M. Humphreys, Gavin Diver, Carl Bartolo, Paulo School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing Engineering Antimicrobial Electrospinning Wounds pose a risk to the skin, our body’s primary defence against infections. The rise of antibiotic resistance has prompted the development of novel therapies. RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide (H2O2), a reactive oxygen species, directly to the wound bed. In this study, electrospinning was used to incorporate RO-101® into a polyvinyl alcohol (PVA) sub-micron fibrous mesh that can act as a delivery agent, achieve a sustained release profile, and provide a barrier against infection. Adequate incorporation of this gel into sub-micron fibres was confirmed via nuclear magnetic resonance spectroscopy. Furthermore, scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200–500 nm range. PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(g·mL) (1 mM = 1 mmol/L) after 24 h, and the role of sterilisation on H2O2 release was evaluated. PVA/RO-101 meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) bacteria, achieving viable count reductions of up to 1 log unit CFU/mm2 (CFU: colony-forming units). Moreover, these meshes were capable of disrupting biofilm formation, even against multidrug-resistant organisms such as methicillin-resistant S. aureus (MRSA). Furthermore, increasing the RO-101® concentration resulted in higher H2O2 production and an enhanced antimicrobial effect, while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101® concentrations. This study therefore demonstrates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings. Published version This work was supported by Matoke Holdings, the United Kingdom (UK) Engineering and Physical Sciences Research Council (EPSRC) Doctoral Prize Fellowship (No. EP/R513131/1), and the Henry Royce Institute for Advanced Materials, funded through EPSRC grants (Nos. EP/R00661X/1, EP/S019367/1, EP/P025021/1, and EP/P025498/1). JYM received financial support from Matoke Holdings. 2025-02-03T07:36:12Z 2025-02-03T07:36:12Z 2024 Journal Article Mieles, J. Y., Vyas, C., Daskalakis, E., Hassan, M., Birkett, J., Omar, A. M., Humphreys, G., Diver, C. & Bartolo, P. (2024). Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release. Bio-Design and Manufacturing, 7(6), 899-925. https://dx.doi.org/10.1007/s42242-024-00312-3 2096-5524 https://hdl.handle.net/10356/182455 10.1007/s42242-024-00312-3 2-s2.0-85208792566 6 7 899 925 en Bio-Design and Manufacturing © 2024 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecomm ons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Engineering Antimicrobial Electrospinning Mieles, Joel Yupanqui Vyas, Cian Daskalakis, Evangelos Hassan, Mohamed Birkett, James Omar, Abdalla M. Humphreys, Gavin Diver, Carl Bartolo, Paulo Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| title | Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| title_full | Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| title_fullStr | Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| title_full_unstemmed | Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| title_short | Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| title_sort | electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release |
| topic | Engineering Antimicrobial Electrospinning |
| url | https://hdl.handle.net/10356/182455 |
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