Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis
MYB family transcription factors (TFs) play crucial roles in plant development, metabolism, and responses to various stresses. However, whether MYB TFs are involved in regulating fatty acid biosynthesis in seeds remains largely elusive. Here, we demonstrated that transgenic Arabidopsis (Arabidopsis...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2025
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| Online Access: | https://hdl.handle.net/10356/182663 |
| _version_ | 1826113575896219648 |
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| author | Yang, Yuzhou Kong, Que Ma, Zhiming Lim, Peng Ken Singh, Sanjay K. Pattanaik, Sitakanta Mutwil, Marek Miao, Yansong Yuan, Ling Ma, Wei |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Yang, Yuzhou Kong, Que Ma, Zhiming Lim, Peng Ken Singh, Sanjay K. Pattanaik, Sitakanta Mutwil, Marek Miao, Yansong Yuan, Ling Ma, Wei |
| author_sort | Yang, Yuzhou |
| collection | NTU |
| description | MYB family transcription factors (TFs) play crucial roles in plant development, metabolism, and responses to various stresses. However, whether MYB TFs are involved in regulating fatty acid biosynthesis in seeds remains largely elusive. Here, we demonstrated that transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing MYB73 exhibit altered FATTY ACID ELONGATION1 (FAE1) expression, seed oil content, and seed fatty acid composition. Electrophoretic mobility shift assays showed that FAE1 is a direct target of MYB73, and functional assays revealed that MYB73 represses FAE1 promoter activity. Transcriptomic analysis of the MYB73-overexpressing plants detected significant changes in the expression of genes involved in fatty acid biosynthesis and triacylglycerol assembly. Furthermore, MYB73 expression was responsive to abscisic acid (ABA), and ABA-responsive element binding factor 2 directly bound to the ABA-responsive element in the MYB73 promoter to activate its expression. Additionally, we determined that MYB73 exhibits the hallmarks of an intrinsically disordered protein and forms phase-separated condensates with liquid-like characteristics, which are important in regulating target gene expression. Together, our findings suggest that MYB73 condensate formation likely fine-tunes seed oil biosynthesis. |
| first_indexed | 2025-03-09T10:53:36Z |
| format | Journal Article |
| id | ntu-10356/182663 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-03-09T10:53:36Z |
| publishDate | 2025 |
| record_format | dspace |
| spelling | ntu-10356/1826632025-02-17T15:32:39Z Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis Yang, Yuzhou Kong, Que Ma, Zhiming Lim, Peng Ken Singh, Sanjay K. Pattanaik, Sitakanta Mutwil, Marek Miao, Yansong Yuan, Ling Ma, Wei School of Biological Sciences Medicine, Health and Life Sciences MYB family transcription factors Plants MYB family transcription factors (TFs) play crucial roles in plant development, metabolism, and responses to various stresses. However, whether MYB TFs are involved in regulating fatty acid biosynthesis in seeds remains largely elusive. Here, we demonstrated that transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing MYB73 exhibit altered FATTY ACID ELONGATION1 (FAE1) expression, seed oil content, and seed fatty acid composition. Electrophoretic mobility shift assays showed that FAE1 is a direct target of MYB73, and functional assays revealed that MYB73 represses FAE1 promoter activity. Transcriptomic analysis of the MYB73-overexpressing plants detected significant changes in the expression of genes involved in fatty acid biosynthesis and triacylglycerol assembly. Furthermore, MYB73 expression was responsive to abscisic acid (ABA), and ABA-responsive element binding factor 2 directly bound to the ABA-responsive element in the MYB73 promoter to activate its expression. Additionally, we determined that MYB73 exhibits the hallmarks of an intrinsically disordered protein and forms phase-separated condensates with liquid-like characteristics, which are important in regulating target gene expression. Together, our findings suggest that MYB73 condensate formation likely fine-tunes seed oil biosynthesis. Ministry of Education (MOE) National Research Foundation (NRF) Published version This work was supported by Ministry of Education (MOE) of Singapore Tier 2 (grant nos. MOE-T2EP30220-0011 and MOE-T2EP30123-0001 to W.M.), MOE of Singapore Tier 1 (grant no. RG32/23 to W.M.), MOE of Singapore Tier 2 (grant no. MOE-T2EP30122-0021 to Y.M.), MOE of Singapore Tier 3 (MOE2019-T3-1-012 to Y.M.), Singapore National Research Foundation Investigatorship (grant no. NRF-NRFI08-2022-0012 to Y.M.), and MOE of Singapore Tier 2 (grant no. MOE- T2EP30122-0017 to M.M.). 2025-02-17T01:05:04Z 2025-02-17T01:05:04Z 2025 Journal Article Yang, Y., Kong, Q., Ma, Z., Lim, P. K., Singh, S. K., Pattanaik, S., Mutwil, M., Miao, Y., Yuan, L. & Ma, W. (2025). Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis. Plant Physiology, 197(2), kiae674-. https://dx.doi.org/10.1093/plphys/kiae674 0032-0889 https://hdl.handle.net/10356/182663 10.1093/plphys/kiae674 39704290 2 197 kiae674 en MOE-T2EP30220-0011 MOE-T2EP30123-0001 RG32/23 MOE-T2EP30122-0021 MOE2019-T3-1-012 NRF-NRFI08-2022-0012 MOET2EP30122-0017 Plant Physiology © 2024 The Author(s). Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/ licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. application/pdf |
| spellingShingle | Medicine, Health and Life Sciences MYB family transcription factors Plants Yang, Yuzhou Kong, Que Ma, Zhiming Lim, Peng Ken Singh, Sanjay K. Pattanaik, Sitakanta Mutwil, Marek Miao, Yansong Yuan, Ling Ma, Wei Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis |
| title | Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis |
| title_full | Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis |
| title_fullStr | Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis |
| title_full_unstemmed | Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis |
| title_short | Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis |
| title_sort | phase separation of myb73 regulates seed oil biosynthesis in arabidopsis |
| topic | Medicine, Health and Life Sciences MYB family transcription factors Plants |
| url | https://hdl.handle.net/10356/182663 |
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