In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application
The objectives of the study are to isolate and serially expand the porcine oesophageal epithelial cells (PEECs) in serum-free, feeder layer-free culture system, to monitor the growth kinetics in in-vitro cultures and to characterize the stem-like nature, proliferative and differentiation potentia...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/18684 |
| _version_ | 1826122554027278336 |
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| author | Thi, Thi Sein. |
| author2 | Chian Kerm Sin |
| author_facet | Chian Kerm Sin Thi, Thi Sein. |
| author_sort | Thi, Thi Sein. |
| collection | NTU |
| description | The objectives of the study are to isolate and serially expand the porcine oesophageal
epithelial cells (PEECs) in serum-free, feeder layer-free culture system, to monitor
the growth kinetics in in-vitro cultures and to characterize the stem-like nature,
proliferative and differentiation potential of cultured PEECs in-vitro with the use of
immunocytochemical method.
The porcine oesophageal epithelial cells were isolated by two-step enzymatic methods using dispase digestion at physiological temperature for earlier establishment of primary cultures. The primary cultures of PEECs were established from porcine oesophageal tissue collected directly from the abattoir. The cells could be cultured in serum-free, feeder layer-free culture medium with reasonable proliferation up to passage 6 – 8 although optimization of culture medium was needed for extended in-vitro growth. Sorting the cells depending on the rapid adhesiveness to extracellular matrix proteins coated on the culture surface could
enrich highly proliferative cells with extended culture lifespan in-vitro.
The morphology of the cultured cells monitored under light microscope confirmed
their morphological stability in in-vitro cultures. The growth kinetics of the cultured
cells was assessed by population doubling time and serial MTS assays. |
| first_indexed | 2024-10-01T05:50:21Z |
| format | Thesis |
| id | ntu-10356/18684 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:50:21Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/186842023-03-11T17:33:14Z In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application Thi, Thi Sein. Chian Kerm Sin School of Mechanical and Aerospace Engineering DRNTU::Science::Medicine::Tissue engineering DRNTU::Science::Medicine::Biomedical engineering The objectives of the study are to isolate and serially expand the porcine oesophageal epithelial cells (PEECs) in serum-free, feeder layer-free culture system, to monitor the growth kinetics in in-vitro cultures and to characterize the stem-like nature, proliferative and differentiation potential of cultured PEECs in-vitro with the use of immunocytochemical method. The porcine oesophageal epithelial cells were isolated by two-step enzymatic methods using dispase digestion at physiological temperature for earlier establishment of primary cultures. The primary cultures of PEECs were established from porcine oesophageal tissue collected directly from the abattoir. The cells could be cultured in serum-free, feeder layer-free culture medium with reasonable proliferation up to passage 6 – 8 although optimization of culture medium was needed for extended in-vitro growth. Sorting the cells depending on the rapid adhesiveness to extracellular matrix proteins coated on the culture surface could enrich highly proliferative cells with extended culture lifespan in-vitro. The morphology of the cultured cells monitored under light microscope confirmed their morphological stability in in-vitro cultures. The growth kinetics of the cultured cells was assessed by population doubling time and serial MTS assays. Master of Science (Biomedical Engineering) 2009-07-02T06:41:14Z 2009-07-02T06:41:14Z 2009 2009 Thesis http://hdl.handle.net/10356/18684 en 116 p. application/pdf |
| spellingShingle | DRNTU::Science::Medicine::Tissue engineering DRNTU::Science::Medicine::Biomedical engineering Thi, Thi Sein. In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application |
| title | In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application |
| title_full | In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application |
| title_fullStr | In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application |
| title_full_unstemmed | In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application |
| title_short | In-vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer-free culture system for tissue engineering application |
| title_sort | in vitro characterization of serially expanded porcine oesophageal epithelial cells in feeder layer free culture system for tissue engineering application |
| topic | DRNTU::Science::Medicine::Tissue engineering DRNTU::Science::Medicine::Biomedical engineering |
| url | http://hdl.handle.net/10356/18684 |
| work_keys_str_mv | AT thithisein invitrocharacterizationofseriallyexpandedporcineoesophagealepithelialcellsinfeederlayerfreeculturesystemfortissueengineeringapplication |