Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins.
Merkel cell polyomavirus (MCV) is the first polyomavirus that can cause human cancer, and was discovered in January of 2008 (Huichen Feng, 2008). Its large T antigen shares structural and sequential similarities with SV40 T antigen and functional similarities with Human Papillomavirus (HPV) E7 pr...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/18936 |
| _version_ | 1811676856892522496 |
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| author | Yu, Esther Dawen. |
| author2 | Kristen Elizabeth Sadler |
| author_facet | Kristen Elizabeth Sadler Yu, Esther Dawen. |
| author_sort | Yu, Esther Dawen. |
| collection | NTU |
| description | Merkel cell polyomavirus (MCV) is the first polyomavirus that can cause human
cancer, and was discovered in January of 2008 (Huichen Feng, 2008). Its large T
antigen shares structural and sequential similarities with SV40 T antigen and
functional similarities with Human Papillomavirus (HPV) E7 proteins. These viral
proteins have tumor transforming ability by binding to the tumor suppressor protein
Retinoblastoma (Rb). In HPV infected cells, E7 binds to Rb and disrupts the Rb-E2F
complex. Accumulation of free E2F then induces p16 expression (Samir N. Khleif, et
al. 1996). Our hypothesis was if MCV and SV40 T antigens use the same
mechanism, we should be able to observe induced p16 expression in cells
transfected with MCV and SV40 T antigen plasmids. The p16 expression in LNCap
cells transfected with SV40 and MCV full-length and C-terminal truncated T antigen
plasmids were detected by immunofluorescence and western blot. Increased p16
expression was only observed in MCV full-length T antigen but not in truncated MCV
T antigen or SV40 T antigen transfected cells. The results might be associated with
different effects of epigenetic modification by MCV and SV40 T antigens. |
| first_indexed | 2024-10-01T02:28:08Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/18936 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:28:08Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/189362023-02-28T18:00:47Z Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. Yu, Esther Dawen. Kristen Elizabeth Sadler School of Biological Sciences Yu, Tsueng Liu DRNTU::Science::Biological sciences::Microbiology::Virology Merkel cell polyomavirus (MCV) is the first polyomavirus that can cause human cancer, and was discovered in January of 2008 (Huichen Feng, 2008). Its large T antigen shares structural and sequential similarities with SV40 T antigen and functional similarities with Human Papillomavirus (HPV) E7 proteins. These viral proteins have tumor transforming ability by binding to the tumor suppressor protein Retinoblastoma (Rb). In HPV infected cells, E7 binds to Rb and disrupts the Rb-E2F complex. Accumulation of free E2F then induces p16 expression (Samir N. Khleif, et al. 1996). Our hypothesis was if MCV and SV40 T antigens use the same mechanism, we should be able to observe induced p16 expression in cells transfected with MCV and SV40 T antigen plasmids. The p16 expression in LNCap cells transfected with SV40 and MCV full-length and C-terminal truncated T antigen plasmids were detected by immunofluorescence and western blot. Increased p16 expression was only observed in MCV full-length T antigen but not in truncated MCV T antigen or SV40 T antigen transfected cells. The results might be associated with different effects of epigenetic modification by MCV and SV40 T antigens. Bachelor of Science in Biological Sciences 2009-08-21T07:17:34Z 2009-08-21T07:17:34Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/18936 en Nanyang Technological University 40 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology Yu, Esther Dawen. Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. |
| title | Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. |
| title_full | Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. |
| title_fullStr | Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. |
| title_full_unstemmed | Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. |
| title_short | Characteristics of Merkel Cell Polyomavirus T antigen oncoproteins. |
| title_sort | characteristics of merkel cell polyomavirus t antigen oncoproteins |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology |
| url | http://hdl.handle.net/10356/18936 |
| work_keys_str_mv | AT yuestherdawen characteristicsofmerkelcellpolyomavirustantigenoncoproteins |