Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant
Metal ions substitution in hydroxyapatite crystal structure is expected to improve biocompatibility and osseointegration to prosthetic implants. Mn(II) and Fe(III) ions substituted hydroxyapatite were synthesized by wet chemical method and ion exchange mechanism with atomic substitution concentrat...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/18954 |
| _version_ | 1811692476838182912 |
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| author | Jasmine Widodo |
| author2 | Lim Sierin |
| author_facet | Lim Sierin Jasmine Widodo |
| author_sort | Jasmine Widodo |
| collection | NTU |
| description | Metal ions substitution in hydroxyapatite crystal structure is expected to improve
biocompatibility and osseointegration to prosthetic implants. Mn(II) and Fe(III) ions
substituted hydroxyapatite were synthesized by wet chemical method and ion exchange mechanism with atomic substitution concentration ranged from 1% - 10% and 1% - 5%, respectively. All samples were single-phased and non-stoichiometric, as indicated by various characterization methods including XRD, FTIR, EDX and ICP.
Biocompatibility testings were done by using human osteoblast ATCC cell line hFOB 1.19. Cytotoxicity test by extraction method and MTT assay showed that Mn(II) and Fe(III) ions had no cytotoxic effect on the osteoblast cells. In addition, it was
established that both Mn(II) and Fe(III) ions improve osteoblast proliferation and
activity (confirmed by ALP assay). The optimum ionic substitution concentration was
observed to be 5% for both Mn(II) and Fe(III) ions. It was believed that higher
metallic ions content led to smaller and less perfect crystal, although not shown in
FESEM images among the atomic substitution percentage here. Significant increase in filopodia formation was observed surrounding osteoblast cells which were seeded on both 5% Mn(II) and Fe(III)-ion-substituted hydroxyapatite pellets, indicating good
cell-substrate attachment, good intercellular interaction, and improved cell viability. |
| first_indexed | 2024-10-01T06:36:24Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/18954 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:36:24Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/189542023-03-03T15:38:00Z Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant Jasmine Widodo Lim Sierin Ooi Chui Ping School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Metal ions substitution in hydroxyapatite crystal structure is expected to improve biocompatibility and osseointegration to prosthetic implants. Mn(II) and Fe(III) ions substituted hydroxyapatite were synthesized by wet chemical method and ion exchange mechanism with atomic substitution concentration ranged from 1% - 10% and 1% - 5%, respectively. All samples were single-phased and non-stoichiometric, as indicated by various characterization methods including XRD, FTIR, EDX and ICP. Biocompatibility testings were done by using human osteoblast ATCC cell line hFOB 1.19. Cytotoxicity test by extraction method and MTT assay showed that Mn(II) and Fe(III) ions had no cytotoxic effect on the osteoblast cells. In addition, it was established that both Mn(II) and Fe(III) ions improve osteoblast proliferation and activity (confirmed by ALP assay). The optimum ionic substitution concentration was observed to be 5% for both Mn(II) and Fe(III) ions. It was believed that higher metallic ions content led to smaller and less perfect crystal, although not shown in FESEM images among the atomic substitution percentage here. Significant increase in filopodia formation was observed surrounding osteoblast cells which were seeded on both 5% Mn(II) and Fe(III)-ion-substituted hydroxyapatite pellets, indicating good cell-substrate attachment, good intercellular interaction, and improved cell viability. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-08-26T01:16:25Z 2009-08-26T01:16:25Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/18954 en Nanyang Technological University 81 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Jasmine Widodo Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant |
| title | Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant |
| title_full | Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant |
| title_fullStr | Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant |
| title_full_unstemmed | Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant |
| title_short | Biocompatibility of metal-ion-substituted hydroxyapatite as orthopaedic/dental implant |
| title_sort | biocompatibility of metal ion substituted hydroxyapatite as orthopaedic dental implant |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/18954 |
| work_keys_str_mv | AT jasminewidodo biocompatibilityofmetalionsubstitutedhydroxyapatiteasorthopaedicdentalimplant |