Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells
Wiskott Aldrich Syndrome (WAS) is a genetic disease caused by recessive mutations in the gene encoding the Wiskott Aldrich Syndrome protein (WASP). Patients suffering from WAS exhibit a variety of symptoms ranging from thrombocytopenia, eczema and immune disorders. WASP belongs to a family of protei...
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| Format: | Thesis |
| Language: | English |
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2009
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| Online Access: | https://hdl.handle.net/10356/20683 |
| _version_ | 1826113955518480384 |
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| author | Raj Mohan Raja Muthiah |
| author2 | Thirumaran s/o Thanabalu |
| author_facet | Thirumaran s/o Thanabalu Raj Mohan Raja Muthiah |
| author_sort | Raj Mohan Raja Muthiah |
| collection | NTU |
| description | Wiskott Aldrich Syndrome (WAS) is a genetic disease caused by recessive mutations in
the gene encoding the Wiskott Aldrich Syndrome protein (WASP). Patients suffering
from WAS exhibit a variety of symptoms ranging from thrombocytopenia, eczema and
immune disorders. WASP belongs to a family of proteins whose members include WASP,
N-WASP, WAVEI-3 and Lasl7p. WASP has a WH1 domain at the N-terminus and the Arp213 complex activating VCA domain at the C-terminus. WASP is expressed predominantly in hematopoietic cells and is involved in integrating extracellular signals and regulating actin cytoskeleton. Lasl7p is the S. cerevisiae ortholog of mammalian WASP and has a similar domain structure as that of WASP. Deletion or mutation in the gene encoding Lasl7p makes the yeast cells inviable at restrictive temperatures and also
causes defects in endocytosis and actin patch polarization at all temperatures. |
| first_indexed | 2024-10-01T03:31:36Z |
| format | Thesis |
| id | ntu-10356/20683 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:31:36Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/206832023-02-28T18:34:35Z Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells Raj Mohan Raja Muthiah Thirumaran s/o Thanabalu School of Biological Sciences DRNTU::Science::Biological sciences Wiskott Aldrich Syndrome (WAS) is a genetic disease caused by recessive mutations in the gene encoding the Wiskott Aldrich Syndrome protein (WASP). Patients suffering from WAS exhibit a variety of symptoms ranging from thrombocytopenia, eczema and immune disorders. WASP belongs to a family of proteins whose members include WASP, N-WASP, WAVEI-3 and Lasl7p. WASP has a WH1 domain at the N-terminus and the Arp213 complex activating VCA domain at the C-terminus. WASP is expressed predominantly in hematopoietic cells and is involved in integrating extracellular signals and regulating actin cytoskeleton. Lasl7p is the S. cerevisiae ortholog of mammalian WASP and has a similar domain structure as that of WASP. Deletion or mutation in the gene encoding Lasl7p makes the yeast cells inviable at restrictive temperatures and also causes defects in endocytosis and actin patch polarization at all temperatures. DOCTOR OF PHILOSOPHY (SBS) 2009-12-23T07:06:03Z 2009-12-23T07:06:03Z 2008 2008 Thesis Raj, M. R. M. (2008). Functional analysis of WASP and Las17p using saccharomyces cerevisiae las17Δ cells. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/20683 10.32657/10356/20683 en 244 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Raj Mohan Raja Muthiah Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells |
| title | Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells |
| title_full | Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells |
| title_fullStr | Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells |
| title_full_unstemmed | Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells |
| title_short | Functional analysis of WASP and Las17p using Saccharomyces cerevisiae las17Δ cells |
| title_sort | functional analysis of wasp and las17p using saccharomyces cerevisiae las17δ cells |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/20683 |
| work_keys_str_mv | AT rajmohanrajamuthiah functionalanalysisofwaspandlas17pusingsaccharomycescerevisiaelas17dcells |