Gene-primer a PCR primer design program
Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reactions. In this project, we develop GENE-PRIMER, a genome- specific PCR primer design program that is amenable to a genome-wide scale. To achieve this, we incorporated various parameters with biological...
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| Format: | Thesis |
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2008
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| Online Access: | http://hdl.handle.net/10356/2674 |
| _version_ | 1824456948784824320 |
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| author | Kok, Wui Lim. |
| author2 | Kwoh, Chee Keong |
| author_facet | Kwoh, Chee Keong Kok, Wui Lim. |
| author_sort | Kok, Wui Lim. |
| collection | NTU |
| description | Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reactions. In this project, we develop GENE-PRIMER, a genome- specific PCR primer design program that is amenable to a genome-wide scale. To achieve this, we incorporated various parameters with biological significance into our program, namely, primer length, melting temperature of primers ,guanine/cytosine (GC) content of primer, homopolymeric runs in primer and self- hybridization tendency of primer. In addition, BLAST algorithm is utilized for the purpose of primer specificity check. In summary, selected primers adhered to both physico-chemical criteria and also display specificity to intended binding site in the genome. |
| first_indexed | 2025-02-19T04:02:13Z |
| format | Thesis |
| id | ntu-10356/2674 |
| institution | Nanyang Technological University |
| last_indexed | 2025-02-19T04:02:13Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | ntu-10356/26742023-03-04T00:31:59Z Gene-primer a PCR primer design program Kok, Wui Lim. Kwoh, Chee Keong School of Computer Engineering DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences Proper PCR primer design determines the success or failure of Polymerase Chain Reaction (PCR) reactions. In this project, we develop GENE-PRIMER, a genome- specific PCR primer design program that is amenable to a genome-wide scale. To achieve this, we incorporated various parameters with biological significance into our program, namely, primer length, melting temperature of primers ,guanine/cytosine (GC) content of primer, homopolymeric runs in primer and self- hybridization tendency of primer. In addition, BLAST algorithm is utilized for the purpose of primer specificity check. In summary, selected primers adhered to both physico-chemical criteria and also display specificity to intended binding site in the genome. Master of Science (Bioinformatics) 2008-09-17T09:07:32Z 2008-09-17T09:07:32Z 2005 2005 Thesis http://hdl.handle.net/10356/2674 Nanyang Technological University application/pdf |
| spellingShingle | DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences Kok, Wui Lim. Gene-primer a PCR primer design program |
| title | Gene-primer a PCR primer design program |
| title_full | Gene-primer a PCR primer design program |
| title_fullStr | Gene-primer a PCR primer design program |
| title_full_unstemmed | Gene-primer a PCR primer design program |
| title_short | Gene-primer a PCR primer design program |
| title_sort | gene primer a pcr primer design program |
| topic | DRNTU::Engineering::Computer science and engineering::Computer applications::Life and medical sciences |
| url | http://hdl.handle.net/10356/2674 |
| work_keys_str_mv | AT kokwuilim geneprimerapcrprimerdesignprogram |