Ceramics-based electrochemical uric acid biosensor
This project explores the development of a novel disposable biosensor based on coating Fe2O3 and Fe3O4 on commercially available screen-printed electrodes. It looks into the preparation, characterisation and measurement of current values from the electrochemical reaction at the biosensor which is us...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2010
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| Online Access: | http://hdl.handle.net/10356/35516 |
| _version_ | 1811689337564168192 |
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| author | Phua, Dindson Jun Liang. |
| author2 | Raju Vijayaraghavan Ramanujan |
| author_facet | Raju Vijayaraghavan Ramanujan Phua, Dindson Jun Liang. |
| author_sort | Phua, Dindson Jun Liang. |
| collection | NTU |
| description | This project explores the development of a novel disposable biosensor based on coating Fe2O3 and Fe3O4 on commercially available screen-printed electrodes. It looks into the preparation, characterisation and measurement of current values from the electrochemical reaction at the biosensor which is useful for the detection of uric acid. The combination of carbon as the substrate, the overlying Fe3O4 layer and the conductive gold coating showed good adsorption properties for the uricase enzyme molecules and yielded good results. 5μl of 5 mg/ml uricase was immobilised onto the electrode surfaces by physical adsorption or physiosorption for a period of 24 hours at 4oC. The electrode was tested against a uric acid concentration of 0.006 mol/L in pH 7.4 Phosphate Buffer Saline (0.1M). It was observed that the current increased by a value of about 0.03 μA, and this presents us with a fair electrochemical response for the detection of uric acid. This approach however can be further refined and improved upon for use in clinical applications and even for detection of other immunological agents. |
| first_indexed | 2024-10-01T05:46:30Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/35516 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:46:30Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/355162023-03-04T15:42:09Z Ceramics-based electrochemical uric acid biosensor Phua, Dindson Jun Liang. Raju Vijayaraghavan Ramanujan School of Materials Science and Engineering DRNTU::Engineering::Materials::Biomaterials This project explores the development of a novel disposable biosensor based on coating Fe2O3 and Fe3O4 on commercially available screen-printed electrodes. It looks into the preparation, characterisation and measurement of current values from the electrochemical reaction at the biosensor which is useful for the detection of uric acid. The combination of carbon as the substrate, the overlying Fe3O4 layer and the conductive gold coating showed good adsorption properties for the uricase enzyme molecules and yielded good results. 5μl of 5 mg/ml uricase was immobilised onto the electrode surfaces by physical adsorption or physiosorption for a period of 24 hours at 4oC. The electrode was tested against a uric acid concentration of 0.006 mol/L in pH 7.4 Phosphate Buffer Saline (0.1M). It was observed that the current increased by a value of about 0.03 μA, and this presents us with a fair electrochemical response for the detection of uric acid. This approach however can be further refined and improved upon for use in clinical applications and even for detection of other immunological agents. Bachelor of Engineering (Materials Engineering) 2010-04-20T03:12:17Z 2010-04-20T03:12:17Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/35516 en Nanyang Technological University 35 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Materials::Biomaterials Phua, Dindson Jun Liang. Ceramics-based electrochemical uric acid biosensor |
| title | Ceramics-based electrochemical uric acid biosensor |
| title_full | Ceramics-based electrochemical uric acid biosensor |
| title_fullStr | Ceramics-based electrochemical uric acid biosensor |
| title_full_unstemmed | Ceramics-based electrochemical uric acid biosensor |
| title_short | Ceramics-based electrochemical uric acid biosensor |
| title_sort | ceramics based electrochemical uric acid biosensor |
| topic | DRNTU::Engineering::Materials::Biomaterials |
| url | http://hdl.handle.net/10356/35516 |
| work_keys_str_mv | AT phuadindsonjunliang ceramicsbasedelectrochemicaluricacidbiosensor |