A microfluidics for life science applications
Despite the vast research on high-throughput platform for parallel genomic assays, a simple yet reliable methodology is still to be discovered. This study investigates one approach of vacuum aided sample loading method to develop PCR-based biochips to analyse thousands of genes in a short time. By p...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2010
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| Online Access: | http://hdl.handle.net/10356/39584 |
| _version_ | 1826122259288293376 |
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| author | An, Shuaichen |
| author2 | Gong Haiqing, Thomas |
| author_facet | Gong Haiqing, Thomas An, Shuaichen |
| author_sort | An, Shuaichen |
| collection | NTU |
| description | Despite the vast research on high-throughput platform for parallel genomic assays, a simple yet reliable methodology is still to be discovered. This study investigates one approach of vacuum aided sample loading method to develop PCR-based biochips to analyse thousands of genes in a short time. By pre-depositing different reagents in the wells, the chip is able to conduct high-throughput assays after the sample loading. Advantages of the chip and method include high well density, low platform cost, simple, low cost operation for end users and high expansibility for automation device development. A systematic research on key parameters has been done. And most parameters show high dependency with chip manufacturing method. High speed camera was used to analysis the internal microfuidic processes aiming to explain phenomena and achieve the optimization of chip design. |
| first_indexed | 2024-10-01T05:45:34Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/39584 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:45:34Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/395842023-03-04T19:28:23Z A microfluidics for life science applications An, Shuaichen Gong Haiqing, Thomas School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering::Fluid mechanics Despite the vast research on high-throughput platform for parallel genomic assays, a simple yet reliable methodology is still to be discovered. This study investigates one approach of vacuum aided sample loading method to develop PCR-based biochips to analyse thousands of genes in a short time. By pre-depositing different reagents in the wells, the chip is able to conduct high-throughput assays after the sample loading. Advantages of the chip and method include high well density, low platform cost, simple, low cost operation for end users and high expansibility for automation device development. A systematic research on key parameters has been done. And most parameters show high dependency with chip manufacturing method. High speed camera was used to analysis the internal microfuidic processes aiming to explain phenomena and achieve the optimization of chip design. Bachelor of Engineering (Mechanical Engineering) 2010-05-31T09:22:44Z 2010-05-31T09:22:44Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39584 en Nanyang Technological University 60 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Mechanical engineering::Fluid mechanics An, Shuaichen A microfluidics for life science applications |
| title | A microfluidics for life science applications |
| title_full | A microfluidics for life science applications |
| title_fullStr | A microfluidics for life science applications |
| title_full_unstemmed | A microfluidics for life science applications |
| title_short | A microfluidics for life science applications |
| title_sort | microfluidics for life science applications |
| topic | DRNTU::Engineering::Mechanical engineering::Fluid mechanics |
| url | http://hdl.handle.net/10356/39584 |
| work_keys_str_mv | AT anshuaichen amicrofluidicsforlifescienceapplications AT anshuaichen microfluidicsforlifescienceapplications |