In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide)
Photo-polymerizable, linear poly(ε-caprolactone-co-lactide-co-glycolide) diacrylates (PCLLGA-DA) are potential candidates for various tissue engineering applications because of their in-vitro biocompatibility, controllable biodegradation rates and non-toxic degradation products. In this project, the...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/39622 |
| _version_ | 1811692765193437184 |
|---|---|
| author | Lim, Jing Yang. |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Lim, Jing Yang. |
| author_sort | Lim, Jing Yang. |
| collection | NTU |
| description | Photo-polymerizable, linear poly(ε-caprolactone-co-lactide-co-glycolide) diacrylates (PCLLGA-DA) are potential candidates for various tissue engineering applications because of their in-vitro biocompatibility, controllable biodegradation rates and non-toxic degradation products. In this project, the biocompatibility evaluation of the formula “H-60” PCLLGA was carried out in a subcutaneous rat model. Polymer disks with diameter of 8mm were prepared and implanted subcutaneously into 9 rat models for 2, 4 and 12 weeks. 2 polymer disks were implanted into each rat model. After the specified period of implantation, the polymer disks were excised for biodegradation and histology studies. The in-vivo degradation profiles of the polymer disks were evaluated by monitoring the average percentage weight loss over the implantation duration. The in-vivo degradation of PCLLGA polymer was found to be faster than in-vitro degradation, with a 9.7% average loss in weight after 12 weeks. For histology study, the polymer disks were viewed under light microscope to evaluate the degree of tissue response. The tissue inflammatory responses were classified as minimal, mild or moderate. After 2 and 4 weeks of implantation duration, minimal inflammatory response was observed as there was a minimal presence of neutrophils and eosinophil. However, moderate inflammatory response after 12 weeks of implantation was found and chronic inflammation, due to the large accumulation of lymphocytes and macrophages was observed. Using a published classification system, “H-60” formula is classified as level 2 biocompatible materials, which could cause a moderate inflammatory response. |
| first_indexed | 2024-10-01T06:40:59Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/39622 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:40:59Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/396222023-03-03T15:38:05Z In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) Lim, Jing Yang. School of Chemical and Biomedical Engineering Singapore Experimental Medicine Centre Professor Mary Chan Bee Eng DRNTU::Engineering::Chemical engineering Photo-polymerizable, linear poly(ε-caprolactone-co-lactide-co-glycolide) diacrylates (PCLLGA-DA) are potential candidates for various tissue engineering applications because of their in-vitro biocompatibility, controllable biodegradation rates and non-toxic degradation products. In this project, the biocompatibility evaluation of the formula “H-60” PCLLGA was carried out in a subcutaneous rat model. Polymer disks with diameter of 8mm were prepared and implanted subcutaneously into 9 rat models for 2, 4 and 12 weeks. 2 polymer disks were implanted into each rat model. After the specified period of implantation, the polymer disks were excised for biodegradation and histology studies. The in-vivo degradation profiles of the polymer disks were evaluated by monitoring the average percentage weight loss over the implantation duration. The in-vivo degradation of PCLLGA polymer was found to be faster than in-vitro degradation, with a 9.7% average loss in weight after 12 weeks. For histology study, the polymer disks were viewed under light microscope to evaluate the degree of tissue response. The tissue inflammatory responses were classified as minimal, mild or moderate. After 2 and 4 weeks of implantation duration, minimal inflammatory response was observed as there was a minimal presence of neutrophils and eosinophil. However, moderate inflammatory response after 12 weeks of implantation was found and chronic inflammation, due to the large accumulation of lymphocytes and macrophages was observed. Using a published classification system, “H-60” formula is classified as level 2 biocompatible materials, which could cause a moderate inflammatory response. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2010-06-01T09:07:04Z 2010-06-01T09:07:04Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39622 en Nanyang Technological University 56 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering Lim, Jing Yang. In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) |
| title | In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) |
| title_full | In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) |
| title_fullStr | In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) |
| title_full_unstemmed | In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) |
| title_short | In vivo biodegradation and biocompatibility of photo-cross-linked film based on poly(ε-caprolactone-co-lactide-co-glycolide) |
| title_sort | in vivo biodegradation and biocompatibility of photo cross linked film based on poly ε caprolactone co lactide co glycolide |
| topic | DRNTU::Engineering::Chemical engineering |
| url | http://hdl.handle.net/10356/39622 |
| work_keys_str_mv | AT limjingyang invivobiodegradationandbiocompatibilityofphotocrosslinkedfilmbasedonpolyecaprolactonecolactidecoglycolide |