Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae
This study focuses on the investigation of effects of light on growth of microalgae Chlorella sp. ATCC 14854. Pre-experimental phase involved medium selection, medium modification and the determination of optimal temperature and pH range suitable for growth. It was found that R-medium was best suite...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2010
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| Online Access: | http://hdl.handle.net/10356/39650 |
| _version_ | 1826128915038470144 |
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| author | Goh, Yvonne Qian Ru. |
| author2 | Lau Wai Man |
| author_facet | Lau Wai Man Goh, Yvonne Qian Ru. |
| author_sort | Goh, Yvonne Qian Ru. |
| collection | NTU |
| description | This study focuses on the investigation of effects of light on growth of microalgae Chlorella sp. ATCC 14854. Pre-experimental phase involved medium selection, medium modification and the determination of optimal temperature and pH range suitable for growth. It was found that R-medium was best suited for growth of Chlorella sp. ATCC 14854; and the optimal temperatures were 28±1°C and pH 6 respectively. R-medium was further modified to yield higher concentrations of iron, magnesium, nitrogen and phosphorus so as to eliminate nutrient limitation or imbalance. Effects of short duration mixing-induced light/dark cycling were studied at four average surface light intensities -- 82μmol/m2.s, 260μmol/m2.s, 370μmol/m2.s and 590μmol/m2.s. Using 82μmol/m2.s, growth performances using both draft tube sizes almost paralleled. However, it was found that the 74mm draft tube runs with a longer cycle time of 1.2s and smaller light fraction of 0.48 outperformed the 54mm draft tube runs with a shorter cycle time of 1.0s and a larger light fraction of 0.72. Results suggested that a light/dark cycling becomes important for higher light fluxes, and longer dark time in relation to light time promoted better growth performance. Lumostatic operations were also investigated based on two light-control parameters—optimal surface light intensity and critical average surface light intensity, where the latter was herein proposed based on investigation of trend of chlorophyll a content. Further work will need to be done to ascertain the superiority of either method. |
| first_indexed | 2024-10-01T07:32:20Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/39650 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:32:20Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/396502023-03-03T15:39:22Z Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae Goh, Yvonne Qian Ru. Lau Wai Man School of Chemical and Biomedical Engineering Lau Wai Man Chen Wei Ning, William DRNTU::Engineering::Chemical engineering::Biotechnology This study focuses on the investigation of effects of light on growth of microalgae Chlorella sp. ATCC 14854. Pre-experimental phase involved medium selection, medium modification and the determination of optimal temperature and pH range suitable for growth. It was found that R-medium was best suited for growth of Chlorella sp. ATCC 14854; and the optimal temperatures were 28±1°C and pH 6 respectively. R-medium was further modified to yield higher concentrations of iron, magnesium, nitrogen and phosphorus so as to eliminate nutrient limitation or imbalance. Effects of short duration mixing-induced light/dark cycling were studied at four average surface light intensities -- 82μmol/m2.s, 260μmol/m2.s, 370μmol/m2.s and 590μmol/m2.s. Using 82μmol/m2.s, growth performances using both draft tube sizes almost paralleled. However, it was found that the 74mm draft tube runs with a longer cycle time of 1.2s and smaller light fraction of 0.48 outperformed the 54mm draft tube runs with a shorter cycle time of 1.0s and a larger light fraction of 0.72. Results suggested that a light/dark cycling becomes important for higher light fluxes, and longer dark time in relation to light time promoted better growth performance. Lumostatic operations were also investigated based on two light-control parameters—optimal surface light intensity and critical average surface light intensity, where the latter was herein proposed based on investigation of trend of chlorophyll a content. Further work will need to be done to ascertain the superiority of either method. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2010-06-02T02:49:08Z 2010-06-02T02:49:08Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39650 en Nanyang Technological University 115 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Goh, Yvonne Qian Ru. Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae |
| title | Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae |
| title_full | Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae |
| title_fullStr | Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae |
| title_full_unstemmed | Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae |
| title_short | Optimization of photobioreactor for production of biodiesel part ii : effect of light on microalgae |
| title_sort | optimization of photobioreactor for production of biodiesel part ii effect of light on microalgae |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/39650 |
| work_keys_str_mv | AT gohyvonneqianru optimizationofphotobioreactorforproductionofbiodieselpartiieffectoflightonmicroalgae |