The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway.
Cell adhesion molecules have long been thought to play an important role in the complex process of adipogenesis. However, little information has been uncovered on the molecular basis of their functions in adipogenesis. The neural cell adhesion molecule (NCAM), a member of the immunoglobulin superfam...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2010
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| Online Access: | http://hdl.handle.net/10356/39667 |
| _version_ | 1811683195301658624 |
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| author | Goh, Kim Jee. |
| author2 | Feng Zhiwei |
| author_facet | Feng Zhiwei Goh, Kim Jee. |
| author_sort | Goh, Kim Jee. |
| collection | NTU |
| description | Cell adhesion molecules have long been thought to play an important role in the complex process of adipogenesis. However, little information has been uncovered on the molecular basis of their functions in adipogenesis. The neural cell adhesion molecule (NCAM), a member of the immunoglobulin superfamily of cell adhesion molecules, was found to play a role in the adipogenesis of murine bone marrow stromal cells (BMSCs). Therefore, the objective of this study is to investigate the molecular mechanisms of how NCAM participates in adipogenesis. Our results show that NCAM-deficient cells possess decreased levels of activated Akt and ERK1/2. While both molecules were found to play important roles in adipogenesis, the inhibition of Akt alone could result in a complete blockade of adipogenesis in BMSCs, indicating its importance in the adipocyte differentiation process. We discovered that the decreased levels of Akt observed in NCAM-deficient mice were due a defect in the insulin signaling pathway, more specifically, at the level of the insulin receptor itself. All in all, our results indicate a role for NCAM in the insulin signaling pathway, one of the major pathways leading to adipogenesis. |
| first_indexed | 2024-10-01T04:08:52Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/39667 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:08:52Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/396672023-02-28T18:00:09Z The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. Goh, Kim Jee. Feng Zhiwei School of Biological Sciences DRNTU::Science::Biological sciences Cell adhesion molecules have long been thought to play an important role in the complex process of adipogenesis. However, little information has been uncovered on the molecular basis of their functions in adipogenesis. The neural cell adhesion molecule (NCAM), a member of the immunoglobulin superfamily of cell adhesion molecules, was found to play a role in the adipogenesis of murine bone marrow stromal cells (BMSCs). Therefore, the objective of this study is to investigate the molecular mechanisms of how NCAM participates in adipogenesis. Our results show that NCAM-deficient cells possess decreased levels of activated Akt and ERK1/2. While both molecules were found to play important roles in adipogenesis, the inhibition of Akt alone could result in a complete blockade of adipogenesis in BMSCs, indicating its importance in the adipocyte differentiation process. We discovered that the decreased levels of Akt observed in NCAM-deficient mice were due a defect in the insulin signaling pathway, more specifically, at the level of the insulin receptor itself. All in all, our results indicate a role for NCAM in the insulin signaling pathway, one of the major pathways leading to adipogenesis. Bachelor of Science in Biological Sciences 2010-06-02T07:05:09Z 2010-06-02T07:05:09Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/39667 en Nanyang Technological University 39 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Goh, Kim Jee. The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. |
| title | The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. |
| title_full | The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. |
| title_fullStr | The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. |
| title_full_unstemmed | The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. |
| title_short | The neural cell adhesion molecule (NCAM) participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway. |
| title_sort | neural cell adhesion molecule ncam participates in the adipogenesis of murine bone marrow stromal cells via the insulin signaling pathway |
| topic | DRNTU::Science::Biological sciences |
| url | http://hdl.handle.net/10356/39667 |
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