Pharmacogenetics of VKORC1 in warfarin resistant patients.
Warfarin is a commonly prescribed anticoagulant that displays wide inter-individual variability in treatment response and dosage. It acts by inhibiting the vitamin K epoxide reductase (VKOR) enzyme in the liver. Genetic variations in vitamin K epoxide reductase complex subunit 1(VKORC1), have been a...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2010
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| Online Access: | http://hdl.handle.net/10356/41759 |
| _version_ | 1826112339201490944 |
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| author | Ong, Jacqueline Kai Mei. |
| author2 | Balram Chowbay |
| author_facet | Balram Chowbay Ong, Jacqueline Kai Mei. |
| author_sort | Ong, Jacqueline Kai Mei. |
| collection | NTU |
| description | Warfarin is a commonly prescribed anticoagulant that displays wide inter-individual variability in treatment response and dosage. It acts by inhibiting the vitamin K epoxide reductase (VKOR) enzyme in the liver. Genetic variations in vitamin K epoxide reductase complex subunit 1(VKORC1), have been associated with differential dose requirement of warfarin. However, high variability in treatment response is still observed. The hypothesis is that other functional polymorphisms in VKORC1 may influence the enzymatic activity of VKOR, leading to variation in the pharmacodynamics of warfarin. Thus, the aim of this study is to investigate novel polymorphisms in VKORC1 that may influence the activity of VKOR in patients receiving varying warfarin dosages. In this study, the VKORC1 gene was screened for novel polymorphisms in 32 subjects. A total of fifteen mutations were found, of which eight were novel. In particular, 337 G>A, 1849_1850 insertion T and 4851 T>G were found to be present in higher frequencies in the high-dose patients. This exploratory study provides the basis for future studies of the pharmacogenetics of VKORC1 in a larger population. |
| first_indexed | 2024-10-01T03:05:23Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/41759 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:05:23Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/417592023-02-28T18:06:44Z Pharmacogenetics of VKORC1 in warfarin resistant patients. Ong, Jacqueline Kai Mei. Balram Chowbay School of Biological Sciences National Cancer Centre Singapore DRNTU::Science::Biological sciences::Microbiology::Drug Resistance Warfarin is a commonly prescribed anticoagulant that displays wide inter-individual variability in treatment response and dosage. It acts by inhibiting the vitamin K epoxide reductase (VKOR) enzyme in the liver. Genetic variations in vitamin K epoxide reductase complex subunit 1(VKORC1), have been associated with differential dose requirement of warfarin. However, high variability in treatment response is still observed. The hypothesis is that other functional polymorphisms in VKORC1 may influence the enzymatic activity of VKOR, leading to variation in the pharmacodynamics of warfarin. Thus, the aim of this study is to investigate novel polymorphisms in VKORC1 that may influence the activity of VKOR in patients receiving varying warfarin dosages. In this study, the VKORC1 gene was screened for novel polymorphisms in 32 subjects. A total of fifteen mutations were found, of which eight were novel. In particular, 337 G>A, 1849_1850 insertion T and 4851 T>G were found to be present in higher frequencies in the high-dose patients. This exploratory study provides the basis for future studies of the pharmacogenetics of VKORC1 in a larger population. Bachelor of Science in Biomedical Sciences 2010-08-11T04:56:25Z 2010-08-11T04:56:25Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/41759 en Nanyang Technological University 27 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Drug Resistance Ong, Jacqueline Kai Mei. Pharmacogenetics of VKORC1 in warfarin resistant patients. |
| title | Pharmacogenetics of VKORC1 in warfarin resistant patients. |
| title_full | Pharmacogenetics of VKORC1 in warfarin resistant patients. |
| title_fullStr | Pharmacogenetics of VKORC1 in warfarin resistant patients. |
| title_full_unstemmed | Pharmacogenetics of VKORC1 in warfarin resistant patients. |
| title_short | Pharmacogenetics of VKORC1 in warfarin resistant patients. |
| title_sort | pharmacogenetics of vkorc1 in warfarin resistant patients |
| topic | DRNTU::Science::Biological sciences::Microbiology::Drug Resistance |
| url | http://hdl.handle.net/10356/41759 |
| work_keys_str_mv | AT ongjacquelinekaimei pharmacogeneticsofvkorc1inwarfarinresistantpatients |