Preparative chiral separations by supercritical fluid chromatography
The overall objective of the project was to develop methods for the rational design of supercritical fluid chromatographic(SFC) processes. There were two significant outcomes of this project: 1. Rational design methodology for SFC separations. 2. A new method for the determination of competitive Lan...
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| Format: | Research Report |
| Language: | English |
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2010
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| Online Access: | http://hdl.handle.net/10356/42252 |
| _version_ | 1811686531091398656 |
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| author | Arvind Rajendran. |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Arvind Rajendran. |
| author_sort | Arvind Rajendran. |
| collection | NTU |
| description | The overall objective of the project was to develop methods for the rational design of supercritical fluid chromatographic(SFC) processes. There were two significant outcomes of this project: 1. Rational design methodology for SFC separations. 2. A new method for the determination of competitive Langmuir isotherm parameters. The separation of the enantiomers of flurbiprofen on an amylose-derived chiral stationary phase, Chiralpak AD-H, by supercritical fluid chromatography under both linear and nonlinear conditions is studied. Pulse injections were implemented using supercritical CO2 modified with methanol as a mobile phase at a temperature of 30◦C. At linear conditions, the isotherm is determined directly from the chromatogram. Under overload conditions, the elution profiles were described by competitive Langmuir and bi-Langmuir isotherm. Isotherm parameters were estimated using the inverse method and the effects of operation variables such as pressure and modifier composition were studied. Optimization algorithms were developed to arrive at conditions to maximize the process performance. Both isocratic and modifier gradient operations were studied.It was shown that by the use of these advanced methods effective separations can be designed and significant improvement in productivity with concurrent reduction in organic solvent consumption can be achieved. These results that show the superior performance of the SFC were experimentally validated. |
| first_indexed | 2024-10-01T05:01:54Z |
| format | Research Report |
| id | ntu-10356/42252 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:01:54Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | ntu-10356/422522023-03-03T15:30:33Z Preparative chiral separations by supercritical fluid chromatography Arvind Rajendran. School of Chemical and Biomedical Engineering DRNTU::Science::Chemistry::Analytical chemistry::Chromatography The overall objective of the project was to develop methods for the rational design of supercritical fluid chromatographic(SFC) processes. There were two significant outcomes of this project: 1. Rational design methodology for SFC separations. 2. A new method for the determination of competitive Langmuir isotherm parameters. The separation of the enantiomers of flurbiprofen on an amylose-derived chiral stationary phase, Chiralpak AD-H, by supercritical fluid chromatography under both linear and nonlinear conditions is studied. Pulse injections were implemented using supercritical CO2 modified with methanol as a mobile phase at a temperature of 30◦C. At linear conditions, the isotherm is determined directly from the chromatogram. Under overload conditions, the elution profiles were described by competitive Langmuir and bi-Langmuir isotherm. Isotherm parameters were estimated using the inverse method and the effects of operation variables such as pressure and modifier composition were studied. Optimization algorithms were developed to arrive at conditions to maximize the process performance. Both isocratic and modifier gradient operations were studied.It was shown that by the use of these advanced methods effective separations can be designed and significant improvement in productivity with concurrent reduction in organic solvent consumption can be achieved. These results that show the superior performance of the SFC were experimentally validated. RG 38/05 2010-10-05T09:10:35Z 2010-10-05T09:10:35Z 2008 2008 Research Report http://hdl.handle.net/10356/42252 en 28 p. application/pdf |
| spellingShingle | DRNTU::Science::Chemistry::Analytical chemistry::Chromatography Arvind Rajendran. Preparative chiral separations by supercritical fluid chromatography |
| title | Preparative chiral separations by supercritical fluid chromatography |
| title_full | Preparative chiral separations by supercritical fluid chromatography |
| title_fullStr | Preparative chiral separations by supercritical fluid chromatography |
| title_full_unstemmed | Preparative chiral separations by supercritical fluid chromatography |
| title_short | Preparative chiral separations by supercritical fluid chromatography |
| title_sort | preparative chiral separations by supercritical fluid chromatography |
| topic | DRNTU::Science::Chemistry::Analytical chemistry::Chromatography |
| url | http://hdl.handle.net/10356/42252 |
| work_keys_str_mv | AT arvindrajendran preparativechiralseparationsbysupercriticalfluidchromatography |