Chemical standaridization and cultivation of traditional Chinese medicine-orchid family

Under this grant, works were carried out to extract and isolate bioactive compounds for chemical standardization of Traditional Chinese Medicine (TCM) orchids such as Gastrodia elata Blume (Orchidaceae)(GE). GE is a notable Chinese medicine and its roots have been widely used for the treatment of ep...

全面介绍

书目详细资料
主要作者: Tan, Swee Ngin.
其他作者: National Institute of Education
格式: Research Report
语言:English
出版: 2010
主题:
在线阅读:http://hdl.handle.net/10356/42320
实物特征
总结:Under this grant, works were carried out to extract and isolate bioactive compounds for chemical standardization of Traditional Chinese Medicine (TCM) orchids such as Gastrodia elata Blume (Orchidaceae)(GE). GE is a notable Chinese medicine and its roots have been widely used for the treatment of epilepsy, headache, rheumatism and vertigo. The major bioactive compounds are gastrodin (GA), vanilly alcohol (VA) and 4-hydroxybenzaldyde (HBA) (Figure 1). Quality, safety and efficacy of herbal medicines are important issues to be addressed toward evidence-based medicine. The analysis of botanicals and herbal preparations involves the combination of extraction methods and analytical techqniues such as separation tools. In the extraction of bioactive compounds from botanicals, laborious and high solvent consumption methods such as heating under reflux and Soxhlet are widely used. In the move to reduce or eliminate use of organic solvents and improve extraction processes, newer sample preparation methods such as pressurized liquid extraction (PLE) and pressurized hot water extraction (PHWE) have been introduced for extraction of bioactive compounds present in plant materials. The aim of the current work was to support the various objectives of RP/04/06TSN, to determine GA and VA present in GE plant materials using PLE at room temperature with aqueous mixture of ethanol. The instrumentation used was based on a low cost laboratory-assembled system (Figure 2). The extraction efficiency of the proposed method was compared with heating under reflux with aqueous mixture of alcohol.