Genetic programs of neuronal degeneration in dendrite arborization neurons.
Pruning is an important neuronal remodeling process that fine-tunes the neuronal circuit and it was found to share similarities with neurodegenerative diseases. However, the underlying mechanisms of pruning remained unknown. Here, we used the Drosophila dorsal dendritic arborization (ddaC) neurons a...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/42731 |
| _version_ | 1826111970369077248 |
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| author | Yap, Kar Wei. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Yap, Kar Wei. |
| author_sort | Yap, Kar Wei. |
| collection | NTU |
| description | Pruning is an important neuronal remodeling process that fine-tunes the neuronal circuit and it was found to share similarities with neurodegenerative diseases. However, the underlying mechanisms of pruning remained unknown. Here, we used the Drosophila dorsal dendritic arborization (ddaC) neurons as a model system to study dendrite pruning. In order to identify genes involved in dendrite pruning, we carried out a genome-wide RNA-mediated gene interference (RNAi) screen using the Gal4-upstream activating sequence (UAS) system. We identified four genes, Female sterile (2) Ketel (Fs(2)Ket), Suppressor of variegation 3-9 (Su(var)3-9), Rpt4 and short wing to be involved in dendrite pruning. Knocking down of these genes resulted in the dendrite pruning defect at 16 hour after puparium formation (APF). Immunostaining results showed that the ecdysone receptor isoform B1 (EcR-B1) level was reduced in the Fs(2)Ket knockdown mutants, suggesting that Fs(2)Ket may work upstream of EcR-B1 signaling. In addition, the downregulated Sox14 and Mical level observed in Fs(2)Ket and Su(var)3-9 knockdown mutants indicated that these two novel genes mediate dendrite pruning by regulating Sox14 and Mical expression. The identification of novel genes helps in elucidating the mechanisms underlying dendrite pruning, possibly aid in the understanding of the molecular pathway of neurodegenerative diseases. |
| first_indexed | 2024-10-01T02:59:14Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/42731 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:59:14Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/427312023-02-28T18:03:43Z Genetic programs of neuronal degeneration in dendrite arborization neurons. Yap, Kar Wei. School of Biological Sciences Yu Fengwei DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Pruning is an important neuronal remodeling process that fine-tunes the neuronal circuit and it was found to share similarities with neurodegenerative diseases. However, the underlying mechanisms of pruning remained unknown. Here, we used the Drosophila dorsal dendritic arborization (ddaC) neurons as a model system to study dendrite pruning. In order to identify genes involved in dendrite pruning, we carried out a genome-wide RNA-mediated gene interference (RNAi) screen using the Gal4-upstream activating sequence (UAS) system. We identified four genes, Female sterile (2) Ketel (Fs(2)Ket), Suppressor of variegation 3-9 (Su(var)3-9), Rpt4 and short wing to be involved in dendrite pruning. Knocking down of these genes resulted in the dendrite pruning defect at 16 hour after puparium formation (APF). Immunostaining results showed that the ecdysone receptor isoform B1 (EcR-B1) level was reduced in the Fs(2)Ket knockdown mutants, suggesting that Fs(2)Ket may work upstream of EcR-B1 signaling. In addition, the downregulated Sox14 and Mical level observed in Fs(2)Ket and Su(var)3-9 knockdown mutants indicated that these two novel genes mediate dendrite pruning by regulating Sox14 and Mical expression. The identification of novel genes helps in elucidating the mechanisms underlying dendrite pruning, possibly aid in the understanding of the molecular pathway of neurodegenerative diseases. Bachelor of Science in Biological Sciences 2011-01-07T08:43:37Z 2011-01-07T08:43:37Z 2010 2010 Final Year Project (FYP) http://hdl.handle.net/10356/42731 en Nanyang Technological University 42 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Yap, Kar Wei. Genetic programs of neuronal degeneration in dendrite arborization neurons. |
| title | Genetic programs of neuronal degeneration in dendrite arborization neurons. |
| title_full | Genetic programs of neuronal degeneration in dendrite arborization neurons. |
| title_fullStr | Genetic programs of neuronal degeneration in dendrite arborization neurons. |
| title_full_unstemmed | Genetic programs of neuronal degeneration in dendrite arborization neurons. |
| title_short | Genetic programs of neuronal degeneration in dendrite arborization neurons. |
| title_sort | genetic programs of neuronal degeneration in dendrite arborization neurons |
| topic | DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology |
| url | http://hdl.handle.net/10356/42731 |
| work_keys_str_mv | AT yapkarwei geneticprogramsofneuronaldegenerationindendritearborizationneurons |