Generation of antibodies towards dengue nonstructural protein 5 using phage display.
Dengue virus (DENV) is the causative agent of dengue infections worldwide, for which no vaccines or antiviral drugs are available at present. The bifunctional nonstructural protein 5 (NS5) is an attractive target for the development of antibodies since it is integral to the replication complex and e...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/44239 |
| _version_ | 1826125820945498112 |
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| author | Koh, Sabrina Rui En. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Koh, Sabrina Rui En. |
| author_sort | Koh, Sabrina Rui En. |
| collection | NTU |
| description | Dengue virus (DENV) is the causative agent of dengue infections worldwide, for which no vaccines or antiviral drugs are available at present. The bifunctional nonstructural protein 5 (NS5) is an attractive target for the development of antibodies since it is integral to the replication complex and essential for DENV replication. In this study, the two functional domains of NS5- methytransferase (MTase) and RNA-dependent RNA polymerase (RdRp) were used as antigens in a multiplex biopanning approach with naïve human Fab-phage display library. Five unique Fab-phage clones were identified; 3 bound to only the RdRp domain, one bound to only the MTase domain and another bound to both domains. These Fab-antibodies have many applications: they are valuable tools that can enable deeper understanding of DENV replication; they are important to drug discovery efforts; and they are potential therapeutics. |
| first_indexed | 2024-10-01T06:42:37Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/44239 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:42:37Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/442392023-02-28T17:59:35Z Generation of antibodies towards dengue nonstructural protein 5 using phage display. Koh, Sabrina Rui En. School of Biological Sciences Duke-NUS Medical School Nicole Moreland Subhash Vasudevan DRNTU::Science::Biological sciences::Microbiology::Virology Dengue virus (DENV) is the causative agent of dengue infections worldwide, for which no vaccines or antiviral drugs are available at present. The bifunctional nonstructural protein 5 (NS5) is an attractive target for the development of antibodies since it is integral to the replication complex and essential for DENV replication. In this study, the two functional domains of NS5- methytransferase (MTase) and RNA-dependent RNA polymerase (RdRp) were used as antigens in a multiplex biopanning approach with naïve human Fab-phage display library. Five unique Fab-phage clones were identified; 3 bound to only the RdRp domain, one bound to only the MTase domain and another bound to both domains. These Fab-antibodies have many applications: they are valuable tools that can enable deeper understanding of DENV replication; they are important to drug discovery efforts; and they are potential therapeutics. Bachelor of Science in Biological Sciences 2011-05-31T06:42:52Z 2011-05-31T06:42:52Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44239 en Nanyang Technological University 37 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology Koh, Sabrina Rui En. Generation of antibodies towards dengue nonstructural protein 5 using phage display. |
| title | Generation of antibodies towards dengue nonstructural protein 5 using phage display. |
| title_full | Generation of antibodies towards dengue nonstructural protein 5 using phage display. |
| title_fullStr | Generation of antibodies towards dengue nonstructural protein 5 using phage display. |
| title_full_unstemmed | Generation of antibodies towards dengue nonstructural protein 5 using phage display. |
| title_short | Generation of antibodies towards dengue nonstructural protein 5 using phage display. |
| title_sort | generation of antibodies towards dengue nonstructural protein 5 using phage display |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology |
| url | http://hdl.handle.net/10356/44239 |
| work_keys_str_mv | AT kohsabrinaruien generationofantibodiestowardsdenguenonstructuralprotein5usingphagedisplay |