Functional role of APP in mediating adult neurogenesis
Amyloid Precursor Protein (APP) signaling pathway was shown to be negatively implicated in embryonic neurogenesis. Hence in this study, the role APP in mediating adult neurogenesis was investigated in vivo using APP wildtype (WT) and knockout (KO) adult mice at the two neurogenic micrcoenvironments-...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/44752 |
| _version_ | 1811695729901568000 |
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| author | Joshva Sundara Raj Jasmine Celina |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Joshva Sundara Raj Jasmine Celina |
| author_sort | Joshva Sundara Raj Jasmine Celina |
| collection | NTU |
| description | Amyloid Precursor Protein (APP) signaling pathway was shown to be negatively implicated in embryonic neurogenesis. Hence in this study, the role APP in mediating adult neurogenesis was investigated in vivo using APP wildtype (WT) and knockout (KO) adult mice at the two neurogenic micrcoenvironments- subventricualar zone (SVZ) of lateral ventricles (LV) and subgranular zone (SGZ) of dentate gyrus (DG). Neural Stem Cell (NSC) proliferation study with 5-bromo-2’-deoxyuridine (BrdU) incorporation at SVZ imply that APP may positively regulate NSC proliferation. Additionally, immuonhistochemistry (IHC) results suggest that APP may also enhance the differentiation of NSCs into olfactory bulb (OB) neurons. Whereas, the findings attained for hippocampal neurogenesis imply that APP may be a negative regulator of differentiation of NSCs into neurons in hippocampus. Though the inconsistencies in the results make it somewhat difficult to draw a profound link that APP might hold with neurogenesis, they do imply to a certain extent that APP impacts neurogenesis perhaps in different ways in the two neurogenic niches. Nonetheless, these results reflect the fact that neurogenesis is constantly subjected to the control of regulatory factors and that APP may exert its regulatory role together with other factors in an orchestrated manner. |
| first_indexed | 2024-10-01T07:28:06Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/44752 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:28:06Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/447522023-02-28T18:04:34Z Functional role of APP in mediating adult neurogenesis Joshva Sundara Raj Jasmine Celina School of Biological Sciences National Neuroscience Institute Zeng Li DRNTU::Science::Biological sciences Amyloid Precursor Protein (APP) signaling pathway was shown to be negatively implicated in embryonic neurogenesis. Hence in this study, the role APP in mediating adult neurogenesis was investigated in vivo using APP wildtype (WT) and knockout (KO) adult mice at the two neurogenic micrcoenvironments- subventricualar zone (SVZ) of lateral ventricles (LV) and subgranular zone (SGZ) of dentate gyrus (DG). Neural Stem Cell (NSC) proliferation study with 5-bromo-2’-deoxyuridine (BrdU) incorporation at SVZ imply that APP may positively regulate NSC proliferation. Additionally, immuonhistochemistry (IHC) results suggest that APP may also enhance the differentiation of NSCs into olfactory bulb (OB) neurons. Whereas, the findings attained for hippocampal neurogenesis imply that APP may be a negative regulator of differentiation of NSCs into neurons in hippocampus. Though the inconsistencies in the results make it somewhat difficult to draw a profound link that APP might hold with neurogenesis, they do imply to a certain extent that APP impacts neurogenesis perhaps in different ways in the two neurogenic niches. Nonetheless, these results reflect the fact that neurogenesis is constantly subjected to the control of regulatory factors and that APP may exert its regulatory role together with other factors in an orchestrated manner. Bachelor of Science in Biological Sciences 2011-06-03T06:55:38Z 2011-06-03T06:55:38Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44752 en Nanyang Technological University 29 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Joshva Sundara Raj Jasmine Celina Functional role of APP in mediating adult neurogenesis |
| title | Functional role of APP in mediating adult neurogenesis |
| title_full | Functional role of APP in mediating adult neurogenesis |
| title_fullStr | Functional role of APP in mediating adult neurogenesis |
| title_full_unstemmed | Functional role of APP in mediating adult neurogenesis |
| title_short | Functional role of APP in mediating adult neurogenesis |
| title_sort | functional role of app in mediating adult neurogenesis |
| topic | DRNTU::Science::Biological sciences |
| url | http://hdl.handle.net/10356/44752 |
| work_keys_str_mv | AT joshvasundararajjasminecelina functionalroleofappinmediatingadultneurogenesis |