Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV).
Human respiratory syncytial virus (HRSV) is a member of Paramyxoviridae family. It is a significant cause of lower respiratory tract infection in babies and children. There are no vaccines available for treatment of HRSV infection at the moment. Thus, the HRSV life cycle and pathogenesis is the s...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/44754 |
| _version_ | 1826121349928583168 |
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| author | Sailov, Talgat. |
| author2 | Jaume Torres |
| author_facet | Jaume Torres Sailov, Talgat. |
| author_sort | Sailov, Talgat. |
| collection | NTU |
| description | Human respiratory syncytial virus (HRSV) is a member of Paramyxoviridae family. It is a
significant cause of lower respiratory tract infection in babies and children. There are no
vaccines available for treatment of HRSV infection at the moment. Thus, the HRSV life cycle
and pathogenesis is the subject of considerable research activity. Small hydrophobic protein
(SH) is an integral membrane protein expressed in HRSV infected cells. Research shows that
SH might play an important role in inhibiting TNF-alpha signaling. Thus, it is a potential drug
target. The goal of the current project is to identify possible interaction partner of SH using yeast
two-hybrid system. Because of hydrophobic nature of SH, its analysis is extremely difficult. Here,
we used Membrane-based Yeast Two-Hybrid system to overcome this problem. Mainly, this
assay utilizes separable domains of ubiquitin to study membrane protein interactions. In this
project, we made a library screening of SH protein and tried to identify the proton conductance
of SH by setting up the liposomal proton flux assay. |
| first_indexed | 2024-10-01T05:31:11Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/44754 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:31:11Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/447542023-02-28T18:06:19Z Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). Sailov, Talgat. Jaume Torres School of Biological Sciences BioSciences Research Centre DRNTU::Science Human respiratory syncytial virus (HRSV) is a member of Paramyxoviridae family. It is a significant cause of lower respiratory tract infection in babies and children. There are no vaccines available for treatment of HRSV infection at the moment. Thus, the HRSV life cycle and pathogenesis is the subject of considerable research activity. Small hydrophobic protein (SH) is an integral membrane protein expressed in HRSV infected cells. Research shows that SH might play an important role in inhibiting TNF-alpha signaling. Thus, it is a potential drug target. The goal of the current project is to identify possible interaction partner of SH using yeast two-hybrid system. Because of hydrophobic nature of SH, its analysis is extremely difficult. Here, we used Membrane-based Yeast Two-Hybrid system to overcome this problem. Mainly, this assay utilizes separable domains of ubiquitin to study membrane protein interactions. In this project, we made a library screening of SH protein and tried to identify the proton conductance of SH by setting up the liposomal proton flux assay. Bachelor of Science in Biological Sciences 2011-06-03T07:01:54Z 2011-06-03T07:01:54Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44754 en Nanyang Technological University 26 p. application/pdf |
| spellingShingle | DRNTU::Science Sailov, Talgat. Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). |
| title | Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). |
| title_full | Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). |
| title_fullStr | Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). |
| title_full_unstemmed | Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). |
| title_short | Application of the split-ubiquitin membrane yeast two-hybrid system to investigate protein interactions of small hydrophobic (SH) protein of human respiratory syncitial virus (HRSV). |
| title_sort | application of the split ubiquitin membrane yeast two hybrid system to investigate protein interactions of small hydrophobic sh protein of human respiratory syncitial virus hrsv |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/44754 |
| work_keys_str_mv | AT sailovtalgat applicationofthesplitubiquitinmembraneyeasttwohybridsystemtoinvestigateproteininteractionsofsmallhydrophobicshproteinofhumanrespiratorysyncitialvirushrsv |