BK virus immunity in patients undergoing hematopoietic stem cell transplant.
Hematopoietic stem cell transplant (HSCT) is an important modality of treatment for hematological diseases and cancers. Latent BK virus (BKV) infection is found in more than 90% of the world’s population. A challenging complication associated with BKV reactivation in HSCT patients is hemorrhagic cys...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/44855 |
| _version_ | 1826125879034511360 |
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| author | Teo, Ai Ling. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Teo, Ai Ling. |
| author_sort | Teo, Ai Ling. |
| collection | NTU |
| description | Hematopoietic stem cell transplant (HSCT) is an important modality of treatment for hematological diseases and cancers. Latent BK virus (BKV) infection is found in more than 90% of the world’s population. A challenging complication associated with BKV reactivation in HSCT patients is hemorrhagic cystitis (HC). There is currently little data about the reconstitution of BKV-specific immunity after HSCT and its relationship to HC. Peripheral blood mononuclear cells (PBMCs) were stimulated with BKV antigens large T and VP1 and the number of BKV-specific T cells were analysed by flow cytometry. Higher levels of CD4+IL-17+ (p<0.01) and CD8+IL-17+ (p=0.05) BKV-specific cells were observed in post engraftment HSCT patients that developed HC compared to those that did not. Furthermore, peak levels of these BKV-specific T cells were observed just before or after the onset of HC and this coincided with high levels of BKV detected in the urine. These preliminary results suggest that BK associated HC is an immunopathology caused by IL-17 secreting BK-specific T cells. Our study will need to be confirmed in a larger cohort of patients. |
| first_indexed | 2024-10-01T06:43:55Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/44855 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:43:55Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/448552023-02-28T18:05:29Z BK virus immunity in patients undergoing hematopoietic stem cell transplant. Teo, Ai Ling. School of Biological Sciences Singapore General Hospital Ling Khoon Lin DRNTU::Science::Biological sciences Hematopoietic stem cell transplant (HSCT) is an important modality of treatment for hematological diseases and cancers. Latent BK virus (BKV) infection is found in more than 90% of the world’s population. A challenging complication associated with BKV reactivation in HSCT patients is hemorrhagic cystitis (HC). There is currently little data about the reconstitution of BKV-specific immunity after HSCT and its relationship to HC. Peripheral blood mononuclear cells (PBMCs) were stimulated with BKV antigens large T and VP1 and the number of BKV-specific T cells were analysed by flow cytometry. Higher levels of CD4+IL-17+ (p<0.01) and CD8+IL-17+ (p=0.05) BKV-specific cells were observed in post engraftment HSCT patients that developed HC compared to those that did not. Furthermore, peak levels of these BKV-specific T cells were observed just before or after the onset of HC and this coincided with high levels of BKV detected in the urine. These preliminary results suggest that BK associated HC is an immunopathology caused by IL-17 secreting BK-specific T cells. Our study will need to be confirmed in a larger cohort of patients. Bachelor of Science in Biological Sciences 2011-06-06T06:23:04Z 2011-06-06T06:23:04Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/44855 en Nanyang Technological University 28 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Teo, Ai Ling. BK virus immunity in patients undergoing hematopoietic stem cell transplant. |
| title | BK virus immunity in patients undergoing hematopoietic stem cell transplant. |
| title_full | BK virus immunity in patients undergoing hematopoietic stem cell transplant. |
| title_fullStr | BK virus immunity in patients undergoing hematopoietic stem cell transplant. |
| title_full_unstemmed | BK virus immunity in patients undergoing hematopoietic stem cell transplant. |
| title_short | BK virus immunity in patients undergoing hematopoietic stem cell transplant. |
| title_sort | bk virus immunity in patients undergoing hematopoietic stem cell transplant |
| topic | DRNTU::Science::Biological sciences |
| url | http://hdl.handle.net/10356/44855 |
| work_keys_str_mv | AT teoailing bkvirusimmunityinpatientsundergoinghematopoieticstemcelltransplant |