Mining biodegradable resource from waste - hunt for PHA microbes
This project was conducted to identify microorganisms which are capable of accumulating polyhydroxyalkanoates (PHAs) by utilizing benzene, toluene, ethylbenzene, p-xylene and styrene (BTEXS) as substrates. Past research has shown that PHA-accumulating microorganisms have been discovered in activa...
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Format: | Final Year Project (FYP) |
Language: | English |
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2011
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Online Access: | http://hdl.handle.net/10356/45020 |
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author | Chong, Wan Ying. |
author2 | Wang Jing-Yuan |
author_facet | Wang Jing-Yuan Chong, Wan Ying. |
author_sort | Chong, Wan Ying. |
collection | NTU |
description | This project was conducted to identify microorganisms which are capable of accumulating polyhydroxyalkanoates (PHAs) by utilizing benzene, toluene, ethylbenzene, p-xylene and styrene (BTEXS) as substrates.
Past research has shown that PHA-accumulating microorganisms have been discovered in activated sludge samples. Therefore, in this study, domestic and industrial activated sludge samples were chosen as potential sources of these microorganisms. Both samples were cultivated under aerobic conditions with BTEXS as the sole carbon source in sequencing batch reactors (SBRs).
BTEXS analysis methods were assessed and they were Ultraviolet-Visible (UV-Vis) spectroscopy, Total Organic Carbon (TOC), Chemical Oxygen Demand (COD) and High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) methods, and it was found that the HPLC-DAD method is most suitable for BTEXS measurements. |
first_indexed | 2024-10-01T04:23:28Z |
format | Final Year Project (FYP) |
id | ntu-10356/45020 |
institution | Nanyang Technological University |
language | English |
last_indexed | 2024-10-01T04:23:28Z |
publishDate | 2011 |
record_format | dspace |
spelling | ntu-10356/450202023-03-03T16:56:19Z Mining biodegradable resource from waste - hunt for PHA microbes Chong, Wan Ying. Wang Jing-Yuan School of Civil and Environmental Engineering DRNTU::Engineering::Environmental engineering::Waste management This project was conducted to identify microorganisms which are capable of accumulating polyhydroxyalkanoates (PHAs) by utilizing benzene, toluene, ethylbenzene, p-xylene and styrene (BTEXS) as substrates. Past research has shown that PHA-accumulating microorganisms have been discovered in activated sludge samples. Therefore, in this study, domestic and industrial activated sludge samples were chosen as potential sources of these microorganisms. Both samples were cultivated under aerobic conditions with BTEXS as the sole carbon source in sequencing batch reactors (SBRs). BTEXS analysis methods were assessed and they were Ultraviolet-Visible (UV-Vis) spectroscopy, Total Organic Carbon (TOC), Chemical Oxygen Demand (COD) and High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) methods, and it was found that the HPLC-DAD method is most suitable for BTEXS measurements. Bachelor of Engineering (Environmental Engineering) 2011-06-08T04:24:50Z 2011-06-08T04:24:50Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45020 en Nanyang Technological University 54 p. application/pdf |
spellingShingle | DRNTU::Engineering::Environmental engineering::Waste management Chong, Wan Ying. Mining biodegradable resource from waste - hunt for PHA microbes |
title | Mining biodegradable resource from waste - hunt for PHA microbes |
title_full | Mining biodegradable resource from waste - hunt for PHA microbes |
title_fullStr | Mining biodegradable resource from waste - hunt for PHA microbes |
title_full_unstemmed | Mining biodegradable resource from waste - hunt for PHA microbes |
title_short | Mining biodegradable resource from waste - hunt for PHA microbes |
title_sort | mining biodegradable resource from waste hunt for pha microbes |
topic | DRNTU::Engineering::Environmental engineering::Waste management |
url | http://hdl.handle.net/10356/45020 |
work_keys_str_mv | AT chongwanying miningbiodegradableresourcefromwastehuntforphamicrobes |