Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer.
To functionally analyze the role of AP-2γ as an ERα cofactor in breast cancer, we focused on examining the phenotypic role of AP-2γ in breast cancer and question if AP-2γ is able to reprogram the transcriptional network of ERα. From our knockdown experiments in MCF-7, we had demonstrated...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2011
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| Online Access: | http://hdl.handle.net/10356/45111 |
| _version_ | 1811679078512590848 |
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| author | Png, Jasmine Jie Min. |
| author2 | Edwin Chong Wing Cheung |
| author_facet | Edwin Chong Wing Cheung Png, Jasmine Jie Min. |
| author_sort | Png, Jasmine Jie Min. |
| collection | NTU |
| description | To functionally analyze the role of AP-2γ as an ERα cofactor in breast cancer, we focused on
examining the phenotypic role of AP-2γ in breast cancer and question if AP-2γ is able to
reprogram the transcriptional network of ERα. From our knockdown experiments in MCF-7,
we had demonstrated that AP-2γ is not involved in cellular proliferation. Instead, it promotes
breast cancer by mediating cell survival. Furthermore, we showed that AP-2γ silencing
resulted in the down-regulation of ERα-upregulated genes, and this was verified by our over-
expression experiments in U2OS Tet-on-ERα, a cell line that lacks AP-2γ expression. Hence,
it can be ascertained that AP-2γ is required for the transcription of ERα-regulated genes.
However, we observed a minimal effect on promoting cell survival after over-expression of
AP-2γ in U2OS Tet-on-ERα. Such phenomenon could be explained by the requirement of
other transcription factors that might cooperate with AP-2γ in the reprogramming machinery.
Despite the partial reprogramming ability, we noticed a significant reduction in cell death in
EtOH-treated U2OS Tet-on-ERα. Therefore, we can suggest that AP-2γ is able to reprogram
the ERα transcription network to a certain extent. |
| first_indexed | 2024-10-01T03:03:26Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/45111 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:03:26Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/451112023-02-28T18:04:10Z Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. Png, Jasmine Jie Min. Edwin Chong Wing Cheung School of Biological Sciences Agency for Science, Technology and Research (A*STAR) DRNTU::Science::Biological sciences::Biochemistry To functionally analyze the role of AP-2γ as an ERα cofactor in breast cancer, we focused on examining the phenotypic role of AP-2γ in breast cancer and question if AP-2γ is able to reprogram the transcriptional network of ERα. From our knockdown experiments in MCF-7, we had demonstrated that AP-2γ is not involved in cellular proliferation. Instead, it promotes breast cancer by mediating cell survival. Furthermore, we showed that AP-2γ silencing resulted in the down-regulation of ERα-upregulated genes, and this was verified by our over- expression experiments in U2OS Tet-on-ERα, a cell line that lacks AP-2γ expression. Hence, it can be ascertained that AP-2γ is required for the transcription of ERα-regulated genes. However, we observed a minimal effect on promoting cell survival after over-expression of AP-2γ in U2OS Tet-on-ERα. Such phenomenon could be explained by the requirement of other transcription factors that might cooperate with AP-2γ in the reprogramming machinery. Despite the partial reprogramming ability, we noticed a significant reduction in cell death in EtOH-treated U2OS Tet-on-ERα. Therefore, we can suggest that AP-2γ is able to reprogram the ERα transcription network to a certain extent. Bachelor of Science in Biological Sciences 2011-06-09T03:17:12Z 2011-06-09T03:17:12Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45111 en Nanyang Technological University 33 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Biochemistry Png, Jasmine Jie Min. Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
| title | Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
| title_full | Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
| title_fullStr | Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
| title_full_unstemmed | Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
| title_short | Functional analysis of AP-2γ as an estrogen receptor α (ERα) cofactor in breast cancer. |
| title_sort | functional analysis of ap 2γ as an estrogen receptor α erα cofactor in breast cancer |
| topic | DRNTU::Science::Biological sciences::Biochemistry |
| url | http://hdl.handle.net/10356/45111 |
| work_keys_str_mv | AT pngjasminejiemin functionalanalysisofap2gasanestrogenreceptoraeracofactorinbreastcancer |