Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1.
Bone marrow stromal cells (BMSCs) have always been attractive targets for clinical cell-based therapies, because of their multipotency and self-renewing abilities. Recently, it has been found that these cells migrate to sites of tissue injury and contribute to repair, however this phenomenon of BMSC...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/45262 |
| _version_ | 1811693466801930240 |
|---|---|
| author | Hang, Yi Ting. |
| author2 | Feng Zhiwei |
| author_facet | Feng Zhiwei Hang, Yi Ting. |
| author_sort | Hang, Yi Ting. |
| collection | NTU |
| description | Bone marrow stromal cells (BMSCs) have always been attractive targets for clinical cell-based therapies, because of their multipotency and self-renewing abilities. Recently, it has been found that these cells migrate to sites of tissue injury and contribute to repair, however this phenomenon of BMSC homing has not been well-characterized. In this project, we hypothesized that NCAM mediates downstream signaling events in response to BMSC migration induced by IGF-1 stimulation. To investigate a possible connection in the roles of these two molecules during BMSC homing, migration assays and western blots were performed; which demonstrated that IGF-1 had a strong stimulatory effect on BMSC migration in vitro. In the absence of NCAM, the ability of IGF-1 to enhance migration was significantly impaired, suggesting that the presence of NCAM is important for the effects of IGF-1. We also found that the level of phosphorylated extracellular signal-regulated kinase (ERK) was increased when NCAM+/+ BMSCs were stimulated with IGF-1. This brings forth the ERK/MAPK pathway as a possible signaling route that is upregulated by NCAM in response to IGF-1 induction. More research could be done on this pathway to determine the molecular basis of IGF-1 signaling through NCAM and hence increase current understanding of BMSC migration. |
| first_indexed | 2024-10-01T06:52:08Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/45262 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:52:08Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/452622023-02-28T18:05:11Z Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. Hang, Yi Ting. Feng Zhiwei School of Biological Sciences DRNTU::Science::Biological sciences::Cytology Bone marrow stromal cells (BMSCs) have always been attractive targets for clinical cell-based therapies, because of their multipotency and self-renewing abilities. Recently, it has been found that these cells migrate to sites of tissue injury and contribute to repair, however this phenomenon of BMSC homing has not been well-characterized. In this project, we hypothesized that NCAM mediates downstream signaling events in response to BMSC migration induced by IGF-1 stimulation. To investigate a possible connection in the roles of these two molecules during BMSC homing, migration assays and western blots were performed; which demonstrated that IGF-1 had a strong stimulatory effect on BMSC migration in vitro. In the absence of NCAM, the ability of IGF-1 to enhance migration was significantly impaired, suggesting that the presence of NCAM is important for the effects of IGF-1. We also found that the level of phosphorylated extracellular signal-regulated kinase (ERK) was increased when NCAM+/+ BMSCs were stimulated with IGF-1. This brings forth the ERK/MAPK pathway as a possible signaling route that is upregulated by NCAM in response to IGF-1 induction. More research could be done on this pathway to determine the molecular basis of IGF-1 signaling through NCAM and hence increase current understanding of BMSC migration. Bachelor of Science in Biological Sciences 2011-06-10T06:09:41Z 2011-06-10T06:09:41Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45262 en Nanyang Technological University 34 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Cytology Hang, Yi Ting. Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. |
| title | Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. |
| title_full | Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. |
| title_fullStr | Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. |
| title_full_unstemmed | Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. |
| title_short | Effects of NCAM on migration in bone marrow stromal cells (BMSCs) induced by IGF-1. |
| title_sort | effects of ncam on migration in bone marrow stromal cells bmscs induced by igf 1 |
| topic | DRNTU::Science::Biological sciences::Cytology |
| url | http://hdl.handle.net/10356/45262 |
| work_keys_str_mv | AT hangyiting effectsofncamonmigrationinbonemarrowstromalcellsbmscsinducedbyigf1 |