The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression.
Human bone marrow-derived mesenchymal stem cells (MSC) have been increasingly used as a vehicle for gene delivery to cancer. Still, little is known about the role of MSC in the tumor microenvironment. Our laboratory has previously observed tumor suppression in glioma-bearing mice co-injected with...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/45269 |
| _version_ | 1824455700116406272 |
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| author | Toh, Xin Yi. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Toh, Xin Yi. |
| author_sort | Toh, Xin Yi. |
| collection | NTU |
| description | Human bone marrow-derived mesenchymal stem cells (MSC) have been increasingly used as a vehicle for gene delivery to cancer. Still, little is known about the role of MSC in the tumor microenvironment. Our laboratory has previously observed tumor suppression in glioma-bearing mice co-injected with human bone marrow-derived mesenchymal stem cells. However, this effect was not observed with PTEN deficient glioma cells. In present study, we employed shRNA as a mean to silence PTEN gene expression (loss-of-function assay). With the observed 26.2% of cell enhancement in MSC/ ∆Gli36-shPTEN co-culture, findings from the loss- of- function assay had indicated that expression of PTEN could be correlated with MSC-mediated suppression. In parallel, isogenic glioma cell lines U251, which overexpresses wild-type PTEN upon tetracycline treatment, resulted in 1.43-fold glioma cell growth retardation when cultured in the presence of MSC. These gain-of-function experiments were in line with the findings in the loss-of-function experiments. In summary, our results had demonstrated the importance of functional PTEN expression in relation to the MSC-mediated tumor cells suppression. |
| first_indexed | 2025-02-19T03:42:22Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/45269 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:42:22Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/452692023-02-28T18:06:55Z The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. Toh, Xin Yi. School of Biological Sciences Paula Lam DRNTU::Science::Biological sciences::Cytology Human bone marrow-derived mesenchymal stem cells (MSC) have been increasingly used as a vehicle for gene delivery to cancer. Still, little is known about the role of MSC in the tumor microenvironment. Our laboratory has previously observed tumor suppression in glioma-bearing mice co-injected with human bone marrow-derived mesenchymal stem cells. However, this effect was not observed with PTEN deficient glioma cells. In present study, we employed shRNA as a mean to silence PTEN gene expression (loss-of-function assay). With the observed 26.2% of cell enhancement in MSC/ ∆Gli36-shPTEN co-culture, findings from the loss- of- function assay had indicated that expression of PTEN could be correlated with MSC-mediated suppression. In parallel, isogenic glioma cell lines U251, which overexpresses wild-type PTEN upon tetracycline treatment, resulted in 1.43-fold glioma cell growth retardation when cultured in the presence of MSC. These gain-of-function experiments were in line with the findings in the loss-of-function experiments. In summary, our results had demonstrated the importance of functional PTEN expression in relation to the MSC-mediated tumor cells suppression. Bachelor of Science in Biological Sciences 2011-06-10T06:28:19Z 2011-06-10T06:28:19Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45269 en Nanyang Technological University 39 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Cytology Toh, Xin Yi. The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. |
| title | The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. |
| title_full | The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. |
| title_fullStr | The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. |
| title_full_unstemmed | The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. |
| title_short | The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. |
| title_sort | role of pten in human bone marrow derived mesenchymal stem cells mediated tumor cells suppression |
| topic | DRNTU::Science::Biological sciences::Cytology |
| url | http://hdl.handle.net/10356/45269 |
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