Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel.
Liver tissue engineering has been an emerging field that shows promising advances to regenerate the liver and treat prevalent liver diseases in order to cope with the shortage of liver donors. Conventional approaches focus on cell-based therapies such as the fabrication of a three-dimensional (3D) l...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2011
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| Online Access: | http://hdl.handle.net/10356/45278 |
| _version_ | 1826125916744450048 |
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| author | Lee, Priscilyn Li Qi. |
| author2 | Wang Dongan |
| author_facet | Wang Dongan Lee, Priscilyn Li Qi. |
| author_sort | Lee, Priscilyn Li Qi. |
| collection | NTU |
| description | Liver tissue engineering has been an emerging field that shows promising advances to regenerate the liver and treat prevalent liver diseases in order to cope with the shortage of liver donors. Conventional approaches focus on cell-based therapies such as the fabrication of a three-dimensional (3D) liver construct to complement cell transplantation and this 3D environment is needed to promote liver cell viability and maintenance of their phenotype. Previous studies have demonstrated a cell delivery composite model with the use of genipin crosslinked gelatin microspheres (GC/GM) as carriers to mediate hepatocellular aggregation, however, with uncontrolled size. In this study, alginate is employed to encapsulate HepG2 laden microspheres in a 3D hydrogel bulk where a collagenase, MMP-9 is introduced to degrade the GC/GM and create well-defined spaces within the alginate to induce spheroid formation. This microcarrier/alginate composite model has allowed for spheroid formation in a size and shape controllable manner where the spheroids take up the size range of the GC/GM (75-160 μm) with a maximum size of 180 μm achieved after two weeks of culture. Spheroids obtained produced higher cell viability and superior functionalities in terms of albumin and urea secretion as well as gene expression of albumin and cytochrome P450. In contrast, HepG2 in control without GC/GM degradation limits growth and has no presence of spheroid. This model not only can be used for generating functional liver spheroids in a large-scale but also can immobilize cell laden microspheres with alginate at the site of injection. |
| first_indexed | 2024-10-01T06:44:14Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/45278 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:44:14Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/452782023-03-03T15:38:07Z Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. Lee, Priscilyn Li Qi. Wang Dongan School of Chemical and Biomedical Engineering DRNTU::Science::Medicine::Tissue engineering DRNTU::Engineering::Materials::Biomaterials Liver tissue engineering has been an emerging field that shows promising advances to regenerate the liver and treat prevalent liver diseases in order to cope with the shortage of liver donors. Conventional approaches focus on cell-based therapies such as the fabrication of a three-dimensional (3D) liver construct to complement cell transplantation and this 3D environment is needed to promote liver cell viability and maintenance of their phenotype. Previous studies have demonstrated a cell delivery composite model with the use of genipin crosslinked gelatin microspheres (GC/GM) as carriers to mediate hepatocellular aggregation, however, with uncontrolled size. In this study, alginate is employed to encapsulate HepG2 laden microspheres in a 3D hydrogel bulk where a collagenase, MMP-9 is introduced to degrade the GC/GM and create well-defined spaces within the alginate to induce spheroid formation. This microcarrier/alginate composite model has allowed for spheroid formation in a size and shape controllable manner where the spheroids take up the size range of the GC/GM (75-160 μm) with a maximum size of 180 μm achieved after two weeks of culture. Spheroids obtained produced higher cell viability and superior functionalities in terms of albumin and urea secretion as well as gene expression of albumin and cytochrome P450. In contrast, HepG2 in control without GC/GM degradation limits growth and has no presence of spheroid. This model not only can be used for generating functional liver spheroids in a large-scale but also can immobilize cell laden microspheres with alginate at the site of injection. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2011-06-10T06:54:14Z 2011-06-10T06:54:14Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45278 en Nanyang Technological University 73 p. application/pdf |
| spellingShingle | DRNTU::Science::Medicine::Tissue engineering DRNTU::Engineering::Materials::Biomaterials Lee, Priscilyn Li Qi. Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. |
| title | Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. |
| title_full | Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. |
| title_fullStr | Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. |
| title_full_unstemmed | Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. |
| title_short | Controlled degradation of HepG2 Laden microspheres induced spheroid formation in alginate hydrogel. |
| title_sort | controlled degradation of hepg2 laden microspheres induced spheroid formation in alginate hydrogel |
| topic | DRNTU::Science::Medicine::Tissue engineering DRNTU::Engineering::Materials::Biomaterials |
| url | http://hdl.handle.net/10356/45278 |
| work_keys_str_mv | AT leepriscilynliqi controlleddegradationofhepg2ladenmicrospheresinducedspheroidformationinalginatehydrogel |