Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex.
The SWI/SNF complex is a large, multi subunit chromatin-remodelling transcriptional regulator complex with an ATPase subunit, Brahma. Recently published data from our laboratory has revealed that Brahma regulates the activity of the EGFR signalling pathway in Drosophila. The study presented here was...
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | |
| التنسيق: | Final Year Project (FYP) |
| اللغة: | English |
| منشور في: |
2011
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10356/46475 |
| _version_ | 1826112204088279040 |
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| author | Siti Nur Ain Roesley. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Siti Nur Ain Roesley. |
| author_sort | Siti Nur Ain Roesley. |
| collection | NTU |
| description | The SWI/SNF complex is a large, multi subunit chromatin-remodelling transcriptional regulator complex with an ATPase subunit, Brahma. Recently published data from our laboratory has revealed that Brahma regulates the activity of the EGFR signalling pathway in Drosophila. The study presented here was conducted to further elucidate which components of the EGFR/Ras/Raf/Erk pathway are regulated by Brahma, and also the mechanism behind this regulation. Analysis of components in this pathway at both the transcriptional and protein levels following overexpression of wild-type Brahma in Drosophila S2 cells, demonstrated that Brahma may affect EGFR mRNA expression and Ras protein level. In addition, previous studies suggest that Brahma activity is regulated through phosphorylation by kinases, including Erk and cyclin E/CDK 2. Analysis of Brahma immunoprecipitated from S2 cells by mass spectrometry revealed contamination by Myosin. Ion exchange chromatography and ammonium sulphate precipitation were used to separate Brahma from Myosin for phosphorylation site analysis by mass spectrometry. |
| first_indexed | 2024-10-01T03:03:27Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/46475 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:03:27Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | ntu-10356/464752023-02-28T18:06:42Z Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. Siti Nur Ain Roesley. School of Biological Sciences Boris Sarcevic DRNTU::Science The SWI/SNF complex is a large, multi subunit chromatin-remodelling transcriptional regulator complex with an ATPase subunit, Brahma. Recently published data from our laboratory has revealed that Brahma regulates the activity of the EGFR signalling pathway in Drosophila. The study presented here was conducted to further elucidate which components of the EGFR/Ras/Raf/Erk pathway are regulated by Brahma, and also the mechanism behind this regulation. Analysis of components in this pathway at both the transcriptional and protein levels following overexpression of wild-type Brahma in Drosophila S2 cells, demonstrated that Brahma may affect EGFR mRNA expression and Ras protein level. In addition, previous studies suggest that Brahma activity is regulated through phosphorylation by kinases, including Erk and cyclin E/CDK 2. Analysis of Brahma immunoprecipitated from S2 cells by mass spectrometry revealed contamination by Myosin. Ion exchange chromatography and ammonium sulphate precipitation were used to separate Brahma from Myosin for phosphorylation site analysis by mass spectrometry. Bachelor of Science in Biological Sciences 2011-12-07T01:17:42Z 2011-12-07T01:17:42Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/46475 en Nanyang Technological University 44 p. application/pdf |
| spellingShingle | DRNTU::Science Siti Nur Ain Roesley. Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. |
| title | Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. |
| title_full | Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. |
| title_fullStr | Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. |
| title_full_unstemmed | Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. |
| title_short | Control of Ras/Erk signalling pathway by the brahma chromatin-remodelling complex. |
| title_sort | control of ras erk signalling pathway by the brahma chromatin remodelling complex |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/46475 |
| work_keys_str_mv | AT sitinurainroesley controlofraserksignallingpathwaybythebrahmachromatinremodellingcomplex |