Regeneratable PCR array chip
The development and applications of polymerase chain reaction(PCR) for DNA detection and quantification are becoming increasingly important in molecular diagnostics and drug development processes. Comparing to conventional PCR instruments, PCR microfluidoc devices, or commonly known as PCR microchip...
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Format: | Thesis |
Language: | English |
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2012
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Online Access: | https://hdl.handle.net/10356/48429 |
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author | Liu, Xiao |
author2 | Gong Haiqing, Thomas |
author_facet | Gong Haiqing, Thomas Liu, Xiao |
author_sort | Liu, Xiao |
collection | NTU |
description | The development and applications of polymerase chain reaction(PCR) for DNA detection and quantification are becoming increasingly important in molecular diagnostics and drug development processes. Comparing to conventional PCR instruments, PCR microfluidoc devices, or commonly known as PCR microchips, have many advantages such as reduced time, reduced sample and reagent consumption, high throughput, etc. However, most of the reported PCR microfluidic devices involved tedious PCR mixture loading process which required an expensive liquid dispensing robot. Furthermore, few efforts have been made to investigate the regenerative capability of PCR microfluidic devices which is crucial in continuous online detection of target samples. Thus a regeneratable PCR array chip was designed and tested in this thesis. |
first_indexed | 2024-10-01T07:09:23Z |
format | Thesis |
id | ntu-10356/48429 |
institution | Nanyang Technological University |
language | English |
last_indexed | 2024-10-01T07:09:23Z |
publishDate | 2012 |
record_format | dspace |
spelling | ntu-10356/484292023-03-11T17:57:09Z Regeneratable PCR array chip Liu, Xiao Gong Haiqing, Thomas School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering The development and applications of polymerase chain reaction(PCR) for DNA detection and quantification are becoming increasingly important in molecular diagnostics and drug development processes. Comparing to conventional PCR instruments, PCR microfluidoc devices, or commonly known as PCR microchips, have many advantages such as reduced time, reduced sample and reagent consumption, high throughput, etc. However, most of the reported PCR microfluidic devices involved tedious PCR mixture loading process which required an expensive liquid dispensing robot. Furthermore, few efforts have been made to investigate the regenerative capability of PCR microfluidic devices which is crucial in continuous online detection of target samples. Thus a regeneratable PCR array chip was designed and tested in this thesis. MASTER OF ENGINEERING (MAE) 2012-04-23T07:08:16Z 2012-04-23T07:08:16Z 2012 2012 Thesis Liu, X. (2012). Regeneratable PCR array chip. Master’s thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/48429 10.32657/10356/48429 en 127 p. application/pdf |
spellingShingle | DRNTU::Engineering::Mechanical engineering Liu, Xiao Regeneratable PCR array chip |
title | Regeneratable PCR array chip |
title_full | Regeneratable PCR array chip |
title_fullStr | Regeneratable PCR array chip |
title_full_unstemmed | Regeneratable PCR array chip |
title_short | Regeneratable PCR array chip |
title_sort | regeneratable pcr array chip |
topic | DRNTU::Engineering::Mechanical engineering |
url | https://hdl.handle.net/10356/48429 |
work_keys_str_mv | AT liuxiao regeneratablepcrarraychip |