"Transcriptional regulation and transcription factors as novel drugs targets for human malaria".
Plasmodium Falciparum has been shown to develop resistance to all classes anti-malarial drugs. More recent cases of resistance to Artemisinin, an effective drug against malaria, have been reported. This will pose a problem as it will be harder to control the spread of malaria. With drug resistance o...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2012
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| Online Access: | http://hdl.handle.net/10356/49258 |
| _version_ | 1811676678606290944 |
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| author | Sakinah Mulyana. |
| author2 | Zbynek Bozdech |
| author_facet | Zbynek Bozdech Sakinah Mulyana. |
| author_sort | Sakinah Mulyana. |
| collection | NTU |
| description | Plasmodium Falciparum has been shown to develop resistance to all classes anti-malarial drugs. More recent cases of resistance to Artemisinin, an effective drug against malaria, have been reported. This will pose a problem as it will be harder to control the spread of malaria. With drug resistance on the rise, there is a need to find new drug targets for the development of new drugs. One possible target is the transcription factor, PF10_0083. In attempt to obtain the protein in its soluble form, a total of 10 constructs were expressed and tested for its solubility after being subjected to different; (i) temperature, (ii) IPTG concentration, (iii) induction time and (iv) different plasmid. Out of the 10 constructs, constructs 225bp and 420bp, seemed to show indication of soluble protein. Further experiments should be done to express the insoluble constructs. For the soluble constructs, the project can progress to the next phase which is to crystallize the protein to further analyze it. |
| first_indexed | 2024-10-01T02:25:18Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49258 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:25:18Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/492582023-02-28T18:06:34Z "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". Sakinah Mulyana. Zbynek Bozdech School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Drug Resistance DRNTU::Science::Biological sciences::Biochemistry Plasmodium Falciparum has been shown to develop resistance to all classes anti-malarial drugs. More recent cases of resistance to Artemisinin, an effective drug against malaria, have been reported. This will pose a problem as it will be harder to control the spread of malaria. With drug resistance on the rise, there is a need to find new drug targets for the development of new drugs. One possible target is the transcription factor, PF10_0083. In attempt to obtain the protein in its soluble form, a total of 10 constructs were expressed and tested for its solubility after being subjected to different; (i) temperature, (ii) IPTG concentration, (iii) induction time and (iv) different plasmid. Out of the 10 constructs, constructs 225bp and 420bp, seemed to show indication of soluble protein. Further experiments should be done to express the insoluble constructs. For the soluble constructs, the project can progress to the next phase which is to crystallize the protein to further analyze it. Bachelor of Science in Biological Sciences 2012-05-16T07:21:49Z 2012-05-16T07:21:49Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49258 en Nanyang Technological University 36 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Drug Resistance DRNTU::Science::Biological sciences::Biochemistry Sakinah Mulyana. "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". |
| title | "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". |
| title_full | "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". |
| title_fullStr | "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". |
| title_full_unstemmed | "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". |
| title_short | "Transcriptional regulation and transcription factors as novel drugs targets for human malaria". |
| title_sort | transcriptional regulation and transcription factors as novel drugs targets for human malaria |
| topic | DRNTU::Science::Biological sciences::Microbiology::Drug Resistance DRNTU::Science::Biological sciences::Biochemistry |
| url | http://hdl.handle.net/10356/49258 |
| work_keys_str_mv | AT sakinahmulyana transcriptionalregulationandtranscriptionfactorsasnoveldrugstargetsforhumanmalaria |