The role of CRTC genes in the development of the zebrafish embryo
CRTC1 (CREB-regulated transcription coactivator 1) is involved in regulation of CREB-target gene expression and transcription activation by MEIS1 (myeloid ecotropic viral integration site 1) protein. Biological functions of CRTC1 have been associated with cancer metastasis, neuronal morphogenesis in...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2012
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| Online Access: | http://hdl.handle.net/10356/49274 |
| _version_ | 1826123066418135040 |
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| author | Fang, Trixy Xinyi |
| author2 | Mark Stephen Featherstone |
| author_facet | Mark Stephen Featherstone Fang, Trixy Xinyi |
| author_sort | Fang, Trixy Xinyi |
| collection | NTU |
| description | CRTC1 (CREB-regulated transcription coactivator 1) is involved in regulation of CREB-target gene expression and transcription activation by MEIS1 (myeloid ecotropic viral integration site 1) protein. Biological functions of CRTC1 have been associated with cancer metastasis, neuronal morphogenesis in mouse and longevity in C.elegans. However, the role of CRTC1 in zebrafish embryonic development has not been characterized yet. Previous unpublished observations showed that morpholino-mediated knockdown of CRTC1 results in a ‘curly tail down’ phenotype of zebrafish morphants. Based on these observations, whole mount in situ hybridization was performed to study muscle-specific and notochord gene expression in crtc1 morphants. Notochords of morphants showed persistent shh
(sonic hedgehog) and ihhb (indian hedgehog homolog b) expression. These results could suggest that CRTC1 is involved in notochord differentiation in the zebrafish embryo. |
| first_indexed | 2024-10-01T05:58:32Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49274 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:58:32Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/492742023-02-28T18:05:37Z The role of CRTC genes in the development of the zebrafish embryo Fang, Trixy Xinyi Mark Stephen Featherstone School of Biological Sciences DRNTU::Science::Biological sciences::Genetics CRTC1 (CREB-regulated transcription coactivator 1) is involved in regulation of CREB-target gene expression and transcription activation by MEIS1 (myeloid ecotropic viral integration site 1) protein. Biological functions of CRTC1 have been associated with cancer metastasis, neuronal morphogenesis in mouse and longevity in C.elegans. However, the role of CRTC1 in zebrafish embryonic development has not been characterized yet. Previous unpublished observations showed that morpholino-mediated knockdown of CRTC1 results in a ‘curly tail down’ phenotype of zebrafish morphants. Based on these observations, whole mount in situ hybridization was performed to study muscle-specific and notochord gene expression in crtc1 morphants. Notochords of morphants showed persistent shh (sonic hedgehog) and ihhb (indian hedgehog homolog b) expression. These results could suggest that CRTC1 is involved in notochord differentiation in the zebrafish embryo. Bachelor of Science in Biological Sciences 2012-05-16T08:32:55Z 2012-05-16T08:32:55Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49274 en Nanyang Technological University 33 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Genetics Fang, Trixy Xinyi The role of CRTC genes in the development of the zebrafish embryo |
| title | The role of CRTC genes in the development of the zebrafish embryo |
| title_full | The role of CRTC genes in the development of the zebrafish embryo |
| title_fullStr | The role of CRTC genes in the development of the zebrafish embryo |
| title_full_unstemmed | The role of CRTC genes in the development of the zebrafish embryo |
| title_short | The role of CRTC genes in the development of the zebrafish embryo |
| title_sort | role of crtc genes in the development of the zebrafish embryo |
| topic | DRNTU::Science::Biological sciences::Genetics |
| url | http://hdl.handle.net/10356/49274 |
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