Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster.
Germline stem cells (GSCs) are adult stem cells known for their ability to produce differentiating gametes that give rise to the next generation. Previous whole-genome microarray expression analysis of Drosophila testis identified the CG3509 gene as a novel candidate stem cell regulator. So f...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2012
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| Online Access: | http://hdl.handle.net/10356/49290 |
| _version_ | 1811690663759052800 |
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| author | Wong, Joyner Yin Sze. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Wong, Joyner Yin Sze. |
| author_sort | Wong, Joyner Yin Sze. |
| collection | NTU |
| description | Germline stem cells (GSCs) are adult stem cells known for their ability to produce differentiating gametes that give rise to the next generation. Previous whole-genome microarray expression analysis of Drosophila testis identified the CG3509 gene as a novel candidate stem cell regulator.
So far, no studies involving CG3509 have been reported. Thus, little is known about CG3509 and its functions in development. To ask what biological functions of CG3509 are during developmental stages, the generation of a loss-of function-allele is required. In this project, I used the P-element excision approach to generate 530 independent excisions of P-element located in 5’untranslated region (UTR) of CG3509. From the 530 independent excisions, I identified one potential CG3509 mutant with a deletion of 169 nucleotides in the intergenic region and 5’UTR without affecting its start codon. The effect of this mutation on CG3509 expression level remains to be tested. Furthermore, I performed immunostaining in wild-type Drosophila embryos with anti-CG3509 antibody newly generated in the laboratory and observed CG3509 signal in the nuclei of both somatic and germline stem cells. However, the specificity of CG3509 staining remains to be tested with the CG3509 loss-of-fuction allele. |
| first_indexed | 2024-10-01T06:07:35Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49290 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:07:35Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/492902023-02-28T18:05:49Z Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. Wong, Joyner Yin Sze. School of Biological Sciences Temasek Laboratories Toshie Kai DRNTU::Science Germline stem cells (GSCs) are adult stem cells known for their ability to produce differentiating gametes that give rise to the next generation. Previous whole-genome microarray expression analysis of Drosophila testis identified the CG3509 gene as a novel candidate stem cell regulator. So far, no studies involving CG3509 have been reported. Thus, little is known about CG3509 and its functions in development. To ask what biological functions of CG3509 are during developmental stages, the generation of a loss-of function-allele is required. In this project, I used the P-element excision approach to generate 530 independent excisions of P-element located in 5’untranslated region (UTR) of CG3509. From the 530 independent excisions, I identified one potential CG3509 mutant with a deletion of 169 nucleotides in the intergenic region and 5’UTR without affecting its start codon. The effect of this mutation on CG3509 expression level remains to be tested. Furthermore, I performed immunostaining in wild-type Drosophila embryos with anti-CG3509 antibody newly generated in the laboratory and observed CG3509 signal in the nuclei of both somatic and germline stem cells. However, the specificity of CG3509 staining remains to be tested with the CG3509 loss-of-fuction allele. Bachelor of Science in Biological Sciences 2012-05-17T01:08:25Z 2012-05-17T01:08:25Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49290 en Nanyang Technological University 38 p. application/pdf |
| spellingShingle | DRNTU::Science Wong, Joyner Yin Sze. Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. |
| title | Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. |
| title_full | Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. |
| title_fullStr | Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. |
| title_full_unstemmed | Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. |
| title_short | Generation of CG3509 mutant allele(s) by p-element excision in Drosophila melanogaster. |
| title_sort | generation of cg3509 mutant allele s by p element excision in drosophila melanogaster |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/49290 |
| work_keys_str_mv | AT wongjoyneryinsze generationofcg3509mutantallelesbypelementexcisionindrosophilamelanogaster |