Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice.
BARP which is endogenously expressed in insulin-secreting cells was found to modulate calcium-dependent secretion through VGCC downregulation. However, little was done to determine the effects of BARP on insulin secretion. Therefore, in addition to previous work on BARP overexpression studies, 12-14...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/10356/49395 |
| _version_ | 1826115127403872256 |
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| author | Tang, Fengxiang. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Tang, Fengxiang. |
| author_sort | Tang, Fengxiang. |
| collection | NTU |
| description | BARP which is endogenously expressed in insulin-secreting cells was found to modulate calcium-dependent secretion through VGCC downregulation. However, little was done to determine the effects of BARP on insulin secretion. Therefore, in addition to previous work on BARP overexpression studies, 12-14-week-old C57BL/6 mice of pancreas-specific BARP knockout (P-BARP-KO) were used here to study the effect of BARP deletion on insulin secretion. Cre recombinase mediated the removal of BARP sequence between loxP sites in P-BARP-KO mice. Effect of BARP deletion was first studied at the systemic level via IPGTT and non-fasting blood tests. P-BARP-KO mice were observed to be glucose intolerant with reduced insulin secretion. Further IPITT analysis dismissed the possibility of insulin insensitivity in P-BARP-KO mice. ELISA measurements of islet insulin content suggested a decrease in insulin production in P-BARP-KO mice. The effect of BARP deletion on islet development was further analyzed through histological observations. General islet architecture of the P-BARP-KO mice appeared normal although morphometric determinations detected reduced β-cell count and β-cell hypertrophy. Therefore, P-BARP-KO mice had reduced plasma insulin with concomitant β-cell changes. Summarizing the previous findings with the current study has shown BARP to assume a regulatory function instead of an essential role in calcium-mediated hormone release. |
| first_indexed | 2024-10-01T03:50:28Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49395 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:50:28Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/493952023-02-28T18:02:20Z Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. Tang, Fengxiang. School of Biological Sciences A*STAR Institute of Molecular and Cell Biology Lim Chiaw Hwee Hunziker Walter DRNTU::Science BARP which is endogenously expressed in insulin-secreting cells was found to modulate calcium-dependent secretion through VGCC downregulation. However, little was done to determine the effects of BARP on insulin secretion. Therefore, in addition to previous work on BARP overexpression studies, 12-14-week-old C57BL/6 mice of pancreas-specific BARP knockout (P-BARP-KO) were used here to study the effect of BARP deletion on insulin secretion. Cre recombinase mediated the removal of BARP sequence between loxP sites in P-BARP-KO mice. Effect of BARP deletion was first studied at the systemic level via IPGTT and non-fasting blood tests. P-BARP-KO mice were observed to be glucose intolerant with reduced insulin secretion. Further IPITT analysis dismissed the possibility of insulin insensitivity in P-BARP-KO mice. ELISA measurements of islet insulin content suggested a decrease in insulin production in P-BARP-KO mice. The effect of BARP deletion on islet development was further analyzed through histological observations. General islet architecture of the P-BARP-KO mice appeared normal although morphometric determinations detected reduced β-cell count and β-cell hypertrophy. Therefore, P-BARP-KO mice had reduced plasma insulin with concomitant β-cell changes. Summarizing the previous findings with the current study has shown BARP to assume a regulatory function instead of an essential role in calcium-mediated hormone release. Bachelor of Science in Biological Sciences 2012-05-18T03:28:54Z 2012-05-18T03:28:54Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49395 en Nanyang Technological University 33 p. application/pdf |
| spellingShingle | DRNTU::Science Tang, Fengxiang. Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. |
| title | Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. |
| title_full | Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. |
| title_fullStr | Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. |
| title_full_unstemmed | Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. |
| title_short | Effect of voltage-gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant C57BL/6 mice. |
| title_sort | effect of voltage gated calcium channel beta anchoring and regulating protein deletion on insulin secretion in glucose intolerant c57bl 6 mice |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/49395 |
| work_keys_str_mv | AT tangfengxiang effectofvoltagegatedcalciumchannelbetaanchoringandregulatingproteindeletiononinsulinsecretioninglucoseintolerantc57bl6mice |