Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis.
The abundance of non-coding RNAs (ncRNAs) and its various roles in human development have brought research closer to understanding the human system beyond the standard central dogma model. One form of ncRNA, the long non-coding RNAs (lncRNAs) has gained increasing importance in human development. Th...
| Үндсэн зохиолч: | |
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| Бусад зохиолчид: | |
| Формат: | Final Year Project (FYP) |
| Хэл сонгох: | English |
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2012
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| Нөхцлүүд: | |
| Онлайн хандалт: | http://hdl.handle.net/10356/49468 |
| _version_ | 1826112983954096128 |
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| author | Lim, Ian-Ling |
| author2 | Surajit Bhattacharyya |
| author_facet | Surajit Bhattacharyya Lim, Ian-Ling |
| author_sort | Lim, Ian-Ling |
| collection | NTU |
| description | The abundance of non-coding RNAs (ncRNAs) and its various roles in human development have brought research closer to understanding the human system beyond the standard central dogma model. One form of ncRNA, the long non-coding RNAs (lncRNAs) has gained increasing importance in human development. The lncRNA of interest is SOX2OT which is an overlapping transcript of the pluripotent transcription factor SOX2. SOX2OT was postulated to regulate SOX2 expression and induce neural progenitor cells (NPCs) during neurogenesis. However, our results showed no correlation for the two cases. Even upon neural induction by Dorsomorphin and SB431542, lower neural expression was seen in SOX2OT overexpressing cells. Furthermore, knock-down of SOX2OT in NPCs did not show any expected acceleration and enhancement in neuron formation as deduced from previous results generated by the lab. Undirected differentiation of human embryonic stem cells (hESCs) overexpressing SOX2OT confirmed poor neuroectodermal and endodermal differentiation efficacy. Instead, the cells differentiated towards the early mesodermal cardiac lineage. As such, though SOX2OT did not seem to play a crucial role in SOX2 regulation and NPC formation, it does affect hESC differentiation and lineage specification during human embryoid development. |
| first_indexed | 2024-10-01T03:15:45Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49468 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:15:45Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/494682023-02-28T18:04:16Z Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. Lim, Ian-Ling Surajit Bhattacharyya School of Biological Sciences A*STAR Genome Institute of Singapore Ng Shi-Yan Stanton Lawrence W. DRNTU::Science The abundance of non-coding RNAs (ncRNAs) and its various roles in human development have brought research closer to understanding the human system beyond the standard central dogma model. One form of ncRNA, the long non-coding RNAs (lncRNAs) has gained increasing importance in human development. The lncRNA of interest is SOX2OT which is an overlapping transcript of the pluripotent transcription factor SOX2. SOX2OT was postulated to regulate SOX2 expression and induce neural progenitor cells (NPCs) during neurogenesis. However, our results showed no correlation for the two cases. Even upon neural induction by Dorsomorphin and SB431542, lower neural expression was seen in SOX2OT overexpressing cells. Furthermore, knock-down of SOX2OT in NPCs did not show any expected acceleration and enhancement in neuron formation as deduced from previous results generated by the lab. Undirected differentiation of human embryonic stem cells (hESCs) overexpressing SOX2OT confirmed poor neuroectodermal and endodermal differentiation efficacy. Instead, the cells differentiated towards the early mesodermal cardiac lineage. As such, though SOX2OT did not seem to play a crucial role in SOX2 regulation and NPC formation, it does affect hESC differentiation and lineage specification during human embryoid development. Bachelor of Science in Biological Sciences 2012-05-21T01:18:40Z 2012-05-21T01:18:40Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49468 en Nanyang Technological University 34 p. application/pdf |
| spellingShingle | DRNTU::Science Lim, Ian-Ling Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. |
| title | Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. |
| title_full | Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. |
| title_fullStr | Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. |
| title_full_unstemmed | Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. |
| title_short | Long non-coding RNA SOX2OT in human embryonic stem cells and neurogenesis. |
| title_sort | long non coding rna sox2ot in human embryonic stem cells and neurogenesis |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/49468 |
| work_keys_str_mv | AT limianling longnoncodingrnasox2otinhumanembryonicstemcellsandneurogenesis |