Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates.
Aggrephagy is a macroautophagy pathway that selectively degrades protein aggregates. Cargo adaptor proteins, NBR1 and p62, have been found to selectively sequester protein aggregates into autophagosome for lysosomal degradation via autophagic pathway. Moreover, the Sphilin-1 protein contains an ANK1...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/10356/49475 |
| _version_ | 1826128594220351488 |
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| author | Tan, Charlene Yan Ning. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Tan, Charlene Yan Ning. |
| author_sort | Tan, Charlene Yan Ning. |
| collection | NTU |
| description | Aggrephagy is a macroautophagy pathway that selectively degrades protein aggregates. Cargo adaptor proteins, NBR1 and p62, have been found to selectively sequester protein aggregates into autophagosome for lysosomal degradation via autophagic pathway. Moreover, the Sphilin-1 protein contains an ANK1 domain that enables it to promote aggrephagy of autophagy non-amendable protein aggregates. However, the basic molecular mechanism behind this degradative pathway is still unknown. Since both Sphilin-1 and cargo adaptors share similar functions in aggrephagy, this study was done to determine if cargo receptors play a role in Sphilin-1 mediated aggrephagy in p62 and NBR1 knockdown cells. Furthermore, additional experiments were conducted in p62 and NBR1 deficient cells to determine their effect on selective autophagy.
Results show that p62 and NBR1 may not be necessary for selective autophagy. Sph-1 mediated clearance of protein aggregates was discovered to be independent of p62 cargo adaptor protein. Together, these findings helps to unravel the mechanistic function of cargo receptors and their role in selective autophagy, be it starvation-induced or Sph-1 mediated. |
| first_indexed | 2024-10-01T07:27:09Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49475 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:27:09Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/494752023-02-28T18:03:35Z Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. Tan, Charlene Yan Ning. School of Biological Sciences Wong Siew Peng Esther DRNTU::Science Aggrephagy is a macroautophagy pathway that selectively degrades protein aggregates. Cargo adaptor proteins, NBR1 and p62, have been found to selectively sequester protein aggregates into autophagosome for lysosomal degradation via autophagic pathway. Moreover, the Sphilin-1 protein contains an ANK1 domain that enables it to promote aggrephagy of autophagy non-amendable protein aggregates. However, the basic molecular mechanism behind this degradative pathway is still unknown. Since both Sphilin-1 and cargo adaptors share similar functions in aggrephagy, this study was done to determine if cargo receptors play a role in Sphilin-1 mediated aggrephagy in p62 and NBR1 knockdown cells. Furthermore, additional experiments were conducted in p62 and NBR1 deficient cells to determine their effect on selective autophagy. Results show that p62 and NBR1 may not be necessary for selective autophagy. Sph-1 mediated clearance of protein aggregates was discovered to be independent of p62 cargo adaptor protein. Together, these findings helps to unravel the mechanistic function of cargo receptors and their role in selective autophagy, be it starvation-induced or Sph-1 mediated. Bachelor of Science in Biological Sciences 2012-05-21T01:39:53Z 2012-05-21T01:39:53Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49475 en Nanyang Technological University 30 p. application/pdf |
| spellingShingle | DRNTU::Science Tan, Charlene Yan Ning. Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. |
| title | Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. |
| title_full | Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. |
| title_fullStr | Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. |
| title_full_unstemmed | Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. |
| title_short | Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates. |
| title_sort | role of cargo receptors in synphilin 1 mediated selective autophagy of protein aggregates |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/49475 |
| work_keys_str_mv | AT tancharleneyanning roleofcargoreceptorsinsynphilin1mediatedselectiveautophagyofproteinaggregates |