Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion.
The most serious form of malaria in humans is caused by the parasite, Plasmodium falciparum. Studies have shown that this parasite recognizes erythrocytes for invasion by binding to receptors on the erythrocyte surface. Other than the availability of receptors, ATP has been shown to be an important...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2012
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| Online Access: | http://hdl.handle.net/10356/49488 |
| _version_ | 1826116350349672448 |
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| author | Loh, Han Ping. |
| author2 | Peter Rainer Preiser |
| author_facet | Peter Rainer Preiser Loh, Han Ping. |
| author_sort | Loh, Han Ping. |
| collection | NTU |
| description | The most serious form of malaria in humans is caused by the parasite, Plasmodium falciparum. Studies have shown that this parasite recognizes erythrocytes for invasion by binding to receptors on the erythrocyte surface. Other than the availability of receptors, ATP has been shown to be an important factor in determining whether an erythrocyte is suitable for invasion. Recent findings that ATP and ADP can bind to a nucleotide binding region (PfRH1-NBR) within Plasmodium falciparum reticulocyte-binding-like protein homologue 1(PfRH1) suggested that PfRH1 might serve as an ATP sensor during invasion. In this project, the biological relevance of ATP in the invasion process was investigated. My results showed that there was a reduction in extracellular ATP during early invasion thereby suggesting that ATP was used in this process. In addition, to further characterize the role of PfRH1-NBR, I had purified monoclonal antibodies that were raised against PfRH1-NBR and found that these antibodies can inhibit invasion. These findings suggested that ATP, through its interaction with PfRH1 is an important factor during parasite invasion. |
| first_indexed | 2024-10-01T04:10:15Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/49488 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:10:15Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/494882023-02-28T18:02:13Z Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. Loh, Han Ping. Peter Rainer Preiser School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Microorganisms The most serious form of malaria in humans is caused by the parasite, Plasmodium falciparum. Studies have shown that this parasite recognizes erythrocytes for invasion by binding to receptors on the erythrocyte surface. Other than the availability of receptors, ATP has been shown to be an important factor in determining whether an erythrocyte is suitable for invasion. Recent findings that ATP and ADP can bind to a nucleotide binding region (PfRH1-NBR) within Plasmodium falciparum reticulocyte-binding-like protein homologue 1(PfRH1) suggested that PfRH1 might serve as an ATP sensor during invasion. In this project, the biological relevance of ATP in the invasion process was investigated. My results showed that there was a reduction in extracellular ATP during early invasion thereby suggesting that ATP was used in this process. In addition, to further characterize the role of PfRH1-NBR, I had purified monoclonal antibodies that were raised against PfRH1-NBR and found that these antibodies can inhibit invasion. These findings suggested that ATP, through its interaction with PfRH1 is an important factor during parasite invasion. Bachelor of Science in Biological Sciences 2012-05-21T02:30:42Z 2012-05-21T02:30:42Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/49488 en Nanyang Technological University 29 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Microorganisms Loh, Han Ping. Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. |
| title | Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. |
| title_full | Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. |
| title_fullStr | Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. |
| title_full_unstemmed | Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. |
| title_short | Insights into the role of the Plasmodium falciparum reticulocyte binding protein RH1 in merozoite invasion. |
| title_sort | insights into the role of the plasmodium falciparum reticulocyte binding protein rh1 in merozoite invasion |
| topic | DRNTU::Science::Biological sciences::Microbiology::Microorganisms |
| url | http://hdl.handle.net/10356/49488 |
| work_keys_str_mv | AT lohhanping insightsintotheroleoftheplasmodiumfalciparumreticulocytebindingproteinrh1inmerozoiteinvasion |