Studies on the host-virus interactions of coronaviruses
Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infect...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | https://hdl.handle.net/10356/49502 |
| _version_ | 1826126822345015296 |
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| author | Zhong, Yanxin |
| author2 | James P. Tam |
| author_facet | James P. Tam Zhong, Yanxin |
| author_sort | Zhong, Yanxin |
| collection | NTU |
| description | Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infected Vero cells by Affymetrix array, using avian coronavirus infectious bronchitis virus (IBV) as a model system, to reveal up-regulation of both pro-apoptotic B cell lymphoma-2 (BCL-2)-antagonist/killer 1 (Bak) and pro-survival myeloid cell leukemia-1 (Mcl-1). Apoptosis occurred earlier in IBV-infected cells silenced with short interfering RNA targeting Mcl-1 (siMcl-1), and was delayed in siBak cells. Up-regulation of RNA helicases from the RIG-I-like receptor (RLR) family, Melanoma differentiation-associated gene 5 (MDA5) and Retinoic-inducible gene I (RIG-I), was also observed in IBV-infected cells, leading to downstream Mitochondrial antiviral signalling (MAVS) adaptor protein activation for interferon induction in response to viral invasion. RIG-I, MDA5 and MAVS also play a part in modulating IBV-induced apoptosis. Bcl-2 family proteins, too, regulates MAVS cleavage during apoptosis, thus further establishing a link between MAVS and intrinsic apoptotic pathway activation in the mitochondria during host innate immunity induction. |
| first_indexed | 2024-10-01T06:58:38Z |
| format | Thesis |
| id | ntu-10356/49502 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:58:38Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/495022023-02-28T18:45:37Z Studies on the host-virus interactions of coronaviruses Zhong, Yanxin James P. Tam Liu Ding Xiang School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infected Vero cells by Affymetrix array, using avian coronavirus infectious bronchitis virus (IBV) as a model system, to reveal up-regulation of both pro-apoptotic B cell lymphoma-2 (BCL-2)-antagonist/killer 1 (Bak) and pro-survival myeloid cell leukemia-1 (Mcl-1). Apoptosis occurred earlier in IBV-infected cells silenced with short interfering RNA targeting Mcl-1 (siMcl-1), and was delayed in siBak cells. Up-regulation of RNA helicases from the RIG-I-like receptor (RLR) family, Melanoma differentiation-associated gene 5 (MDA5) and Retinoic-inducible gene I (RIG-I), was also observed in IBV-infected cells, leading to downstream Mitochondrial antiviral signalling (MAVS) adaptor protein activation for interferon induction in response to viral invasion. RIG-I, MDA5 and MAVS also play a part in modulating IBV-induced apoptosis. Bcl-2 family proteins, too, regulates MAVS cleavage during apoptosis, thus further establishing a link between MAVS and intrinsic apoptotic pathway activation in the mitochondria during host innate immunity induction. DOCTOR OF PHILOSOPHY (SBS) 2012-05-21T04:04:18Z 2012-05-21T04:04:18Z 2012 2012 Thesis Zhong, Y. (2012). Studies on the host-virus interactions of coronaviruses. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/49502 10.32657/10356/49502 en 217 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Virology Zhong, Yanxin Studies on the host-virus interactions of coronaviruses |
| title | Studies on the host-virus interactions of coronaviruses |
| title_full | Studies on the host-virus interactions of coronaviruses |
| title_fullStr | Studies on the host-virus interactions of coronaviruses |
| title_full_unstemmed | Studies on the host-virus interactions of coronaviruses |
| title_short | Studies on the host-virus interactions of coronaviruses |
| title_sort | studies on the host virus interactions of coronaviruses |
| topic | DRNTU::Science::Biological sciences::Microbiology::Virology |
| url | https://hdl.handle.net/10356/49502 |
| work_keys_str_mv | AT zhongyanxin studiesonthehostvirusinteractionsofcoronaviruses |