Development of a micro flow control system

PCR (Polymerase Chain Reaction) is a useful technique which is very common use in current. PCR is also a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Said...

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Bibliographic Details
Main Author: Tee, Yi Fei.
Other Authors: Gong Haiqing, Thomas
Format: Final Year Project (FYP)
Language:English
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10356/50335
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author Tee, Yi Fei.
author2 Gong Haiqing, Thomas
author_facet Gong Haiqing, Thomas
Tee, Yi Fei.
author_sort Tee, Yi Fei.
collection NTU
description PCR (Polymerase Chain Reaction) is a useful technique which is very common use in current. PCR is also a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Said in simple way is to duplicate the DNA strands using this technique. In pre-existing 3'-OH group, the DNA polymerase can add a nucleotide only, so it needs a primer to which it can add the first nucleotide. At the end of the PCR reaction process, the specific sequence will be accumulated in billions of copies (amplicons).
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spelling ntu-10356/503352023-03-04T18:23:25Z Development of a micro flow control system Tee, Yi Fei. Gong Haiqing, Thomas School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering PCR (Polymerase Chain Reaction) is a useful technique which is very common use in current. PCR is also a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Said in simple way is to duplicate the DNA strands using this technique. In pre-existing 3'-OH group, the DNA polymerase can add a nucleotide only, so it needs a primer to which it can add the first nucleotide. At the end of the PCR reaction process, the specific sequence will be accumulated in billions of copies (amplicons). Bachelor of Engineering (Mechanical Engineering) 2012-06-01T02:15:48Z 2012-06-01T02:15:48Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/50335 en Nanyang Technological University 69 p. application/pdf
spellingShingle DRNTU::Engineering::Mechanical engineering
Tee, Yi Fei.
Development of a micro flow control system
title Development of a micro flow control system
title_full Development of a micro flow control system
title_fullStr Development of a micro flow control system
title_full_unstemmed Development of a micro flow control system
title_short Development of a micro flow control system
title_sort development of a micro flow control system
topic DRNTU::Engineering::Mechanical engineering
url http://hdl.handle.net/10356/50335
work_keys_str_mv AT teeyifei developmentofamicroflowcontrolsystem