Development of a micro flow control system
PCR (Polymerase Chain Reaction) is a useful technique which is very common use in current. PCR is also a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Said...
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Format: | Final Year Project (FYP) |
Language: | English |
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2012
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Online Access: | http://hdl.handle.net/10356/50335 |
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author | Tee, Yi Fei. |
author2 | Gong Haiqing, Thomas |
author_facet | Gong Haiqing, Thomas Tee, Yi Fei. |
author_sort | Tee, Yi Fei. |
collection | NTU |
description | PCR (Polymerase Chain Reaction) is a useful technique which is very common use in current. PCR is also a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Said in simple way is to duplicate the DNA strands using this technique. In pre-existing 3'-OH group, the DNA polymerase can add a nucleotide only, so it needs a primer to which it can add the first nucleotide. At the end of the PCR reaction process, the specific sequence will be accumulated in billions of copies (amplicons). |
first_indexed | 2024-10-01T02:54:23Z |
format | Final Year Project (FYP) |
id | ntu-10356/50335 |
institution | Nanyang Technological University |
language | English |
last_indexed | 2024-10-01T02:54:23Z |
publishDate | 2012 |
record_format | dspace |
spelling | ntu-10356/503352023-03-04T18:23:25Z Development of a micro flow control system Tee, Yi Fei. Gong Haiqing, Thomas School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering PCR (Polymerase Chain Reaction) is a useful technique which is very common use in current. PCR is also a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Said in simple way is to duplicate the DNA strands using this technique. In pre-existing 3'-OH group, the DNA polymerase can add a nucleotide only, so it needs a primer to which it can add the first nucleotide. At the end of the PCR reaction process, the specific sequence will be accumulated in billions of copies (amplicons). Bachelor of Engineering (Mechanical Engineering) 2012-06-01T02:15:48Z 2012-06-01T02:15:48Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/50335 en Nanyang Technological University 69 p. application/pdf |
spellingShingle | DRNTU::Engineering::Mechanical engineering Tee, Yi Fei. Development of a micro flow control system |
title | Development of a micro flow control system |
title_full | Development of a micro flow control system |
title_fullStr | Development of a micro flow control system |
title_full_unstemmed | Development of a micro flow control system |
title_short | Development of a micro flow control system |
title_sort | development of a micro flow control system |
topic | DRNTU::Engineering::Mechanical engineering |
url | http://hdl.handle.net/10356/50335 |
work_keys_str_mv | AT teeyifei developmentofamicroflowcontrolsystem |