Age-related structural and compositional variations of mouse skeletal muscle
Skeletal muscles undergo loss of mass and strength during aging, known as sarcopenia. Sarcopenia impairs skeletal muscle function and is characterized by a series of structural and compositional variations. In this project, I first verified that a reduced myofibre volume, i.e. atrophy, was associate...
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| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | https://hdl.handle.net/10356/50487 |
| _version_ | 1826122184130560000 |
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| author | Wang, Huijuan |
| author2 | Lee Kijoon |
| author_facet | Lee Kijoon Wang, Huijuan |
| author_sort | Wang, Huijuan |
| collection | NTU |
| description | Skeletal muscles undergo loss of mass and strength during aging, known as sarcopenia. Sarcopenia impairs skeletal muscle function and is characterized by a series of structural and compositional variations. In this project, I first verified that a reduced myofibre volume, i.e. atrophy, was associated with modifications in myofibre morphology. I indicated that the quantity of myonuclei (per myofibre) maintained during aging. I also reported an increased myonuclear centralization, which suggested that in the old muscle, myofibres were still capable of being regenerated by satellite cells. Although it seems well known that myonuclei yielded from a balance between their regeneration and their apoptosis, I revealed that myonuclear apoptosis was a rare event even in the old muscle. I further investigated the distribution of muscle apoptosis and noticed that most apoptosis occurred in capillary endothelial cells. Such capillary apoptosis may trigger important molecular events, i.e. cleavage of endorepellin fragments, which have been reported to stimulate fibrosis. |
| first_indexed | 2024-10-01T05:44:19Z |
| format | Thesis |
| id | ntu-10356/50487 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:44:19Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/504872023-03-03T16:03:00Z Age-related structural and compositional variations of mouse skeletal muscle Wang, Huijuan Lee Kijoon School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Skeletal muscles undergo loss of mass and strength during aging, known as sarcopenia. Sarcopenia impairs skeletal muscle function and is characterized by a series of structural and compositional variations. In this project, I first verified that a reduced myofibre volume, i.e. atrophy, was associated with modifications in myofibre morphology. I indicated that the quantity of myonuclei (per myofibre) maintained during aging. I also reported an increased myonuclear centralization, which suggested that in the old muscle, myofibres were still capable of being regenerated by satellite cells. Although it seems well known that myonuclei yielded from a balance between their regeneration and their apoptosis, I revealed that myonuclear apoptosis was a rare event even in the old muscle. I further investigated the distribution of muscle apoptosis and noticed that most apoptosis occurred in capillary endothelial cells. Such capillary apoptosis may trigger important molecular events, i.e. cleavage of endorepellin fragments, which have been reported to stimulate fibrosis. DOCTOR OF PHILOSOPHY (SCBE) 2012-06-06T02:46:30Z 2012-06-06T02:46:30Z 2012 2012 Thesis Wang, H. J. (2012). Age-related structural and compositional variations of mouse skeletal muscle. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/50487 10.32657/10356/50487 en 185 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Wang, Huijuan Age-related structural and compositional variations of mouse skeletal muscle |
| title | Age-related structural and compositional variations of mouse skeletal muscle |
| title_full | Age-related structural and compositional variations of mouse skeletal muscle |
| title_fullStr | Age-related structural and compositional variations of mouse skeletal muscle |
| title_full_unstemmed | Age-related structural and compositional variations of mouse skeletal muscle |
| title_short | Age-related structural and compositional variations of mouse skeletal muscle |
| title_sort | age related structural and compositional variations of mouse skeletal muscle |
| topic | DRNTU::Engineering::Bioengineering |
| url | https://hdl.handle.net/10356/50487 |
| work_keys_str_mv | AT wanghuijuan agerelatedstructuralandcompositionalvariationsofmouseskeletalmuscle |