Development of an immobilization-capture method for optical detection of bacteria
There is a need to develop an online bacterial detection system which enables sample preparation, concentration and bacterial detection to be done in realtime. A flow immunosensor approach based on detection of the fluorescence emission has high potential to be developed into an online bacterial det...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/10356/50506 |
| _version_ | 1811681852278177792 |
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| author | Er, Chin Lu |
| author2 | Sun Delai, Darren |
| author_facet | Sun Delai, Darren Er, Chin Lu |
| author_sort | Er, Chin Lu |
| collection | NTU |
| description | There is a need to develop an online bacterial detection system which enables sample preparation, concentration and bacterial detection to be done in realtime. A flow immunosensor approach based on detection of the fluorescence emission has high potential to be developed into an online bacterial detector. In this study, we developed a system of immobilizing fluorescently-labelled antibody onto a glass surface where the cells are captured.
This study aimed to develop an antibody immobilization method to
immobilize fluorescently-labelled antibody specific to the target bacteria (E.coli)
onto the glass surface to capture and label the cells. X-ray photoelectron
spectroscopy (XPS), atomic force microscopy (AFM) and epifluorescence
microscopy studies revealed the feasibility of using aminopropyltriethoxysilane-glutaraldehyde
(APTES-GA) to immobilize antibody onto glass substrates. |
| first_indexed | 2024-10-01T03:47:31Z |
| format | Thesis |
| id | ntu-10356/50506 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:47:31Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/505062023-03-03T19:14:03Z Development of an immobilization-capture method for optical detection of bacteria Er, Chin Lu Sun Delai, Darren School of Civil and Environmental Engineering DRNTU::Engineering::Environmental engineering There is a need to develop an online bacterial detection system which enables sample preparation, concentration and bacterial detection to be done in realtime. A flow immunosensor approach based on detection of the fluorescence emission has high potential to be developed into an online bacterial detector. In this study, we developed a system of immobilizing fluorescently-labelled antibody onto a glass surface where the cells are captured. This study aimed to develop an antibody immobilization method to immobilize fluorescently-labelled antibody specific to the target bacteria (E.coli) onto the glass surface to capture and label the cells. X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and epifluorescence microscopy studies revealed the feasibility of using aminopropyltriethoxysilane-glutaraldehyde (APTES-GA) to immobilize antibody onto glass substrates. Master of Engineering (CEE) 2012-06-08T03:25:01Z 2012-06-08T03:25:01Z 2012 2012 Thesis Er, C. L. (2012). Development of an immobilization-capture method for optical detection of bacteria. Master’s thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/50506 en 108 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering Er, Chin Lu Development of an immobilization-capture method for optical detection of bacteria |
| title | Development of an immobilization-capture method for optical detection of bacteria |
| title_full | Development of an immobilization-capture method for optical detection of bacteria |
| title_fullStr | Development of an immobilization-capture method for optical detection of bacteria |
| title_full_unstemmed | Development of an immobilization-capture method for optical detection of bacteria |
| title_short | Development of an immobilization-capture method for optical detection of bacteria |
| title_sort | development of an immobilization capture method for optical detection of bacteria |
| topic | DRNTU::Engineering::Environmental engineering |
| url | http://hdl.handle.net/10356/50506 |
| work_keys_str_mv | AT erchinlu developmentofanimmobilizationcapturemethodforopticaldetectionofbacteria |