Metabolic changes of host cells in response to HBV replication
As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and q...
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | http://hdl.handle.net/10356/50533 |
| _version_ | 1826118549141192704 |
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| author | Li, Lingyu |
| author2 | Chen Wei Ning, William |
| author_facet | Chen Wei Ning, William Li, Lingyu |
| author_sort | Li, Lingyu |
| collection | NTU |
| description | As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and quantitatively analyse the metabolites change of host cell in response to the introduction of HBx gene. Four kinds of metabolites are finally picked out: butanedioic acid, glutamine, lactic acid and D-glucose. The first two molecules are significantly down-regulated while the other two seem to be up-regulated, suggesting that glycolysis of the infected cells is enhanced, while TCAC activity is inhibited. |
| first_indexed | 2024-10-01T04:45:16Z |
| format | Thesis |
| id | ntu-10356/50533 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:45:16Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/505332023-03-03T16:01:36Z Metabolic changes of host cells in response to HBV replication Li, Lingyu Chen Wei Ning, William School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering As a multifunctional regulator, HBx has long been suspected as the primary factor for inducing HCC. However, as a DNA virus, HBV exhibits relatively high mutation rate, thus studying the pathology of different genotypes of HBx mutants seems to be significant. GC/MS is utilized to qualitatively and quantitatively analyse the metabolites change of host cell in response to the introduction of HBx gene. Four kinds of metabolites are finally picked out: butanedioic acid, glutamine, lactic acid and D-glucose. The first two molecules are significantly down-regulated while the other two seem to be up-regulated, suggesting that glycolysis of the infected cells is enhanced, while TCAC activity is inhibited. Master of Science 2012-06-11T09:01:14Z 2012-06-11T09:01:14Z 2012 2012 Thesis http://hdl.handle.net/10356/50533 en 53 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering Li, Lingyu Metabolic changes of host cells in response to HBV replication |
| title | Metabolic changes of host cells in response to HBV replication |
| title_full | Metabolic changes of host cells in response to HBV replication |
| title_fullStr | Metabolic changes of host cells in response to HBV replication |
| title_full_unstemmed | Metabolic changes of host cells in response to HBV replication |
| title_short | Metabolic changes of host cells in response to HBV replication |
| title_sort | metabolic changes of host cells in response to hbv replication |
| topic | DRNTU::Engineering::Chemical engineering |
| url | http://hdl.handle.net/10356/50533 |
| work_keys_str_mv | AT lilingyu metabolicchangesofhostcellsinresponsetohbvreplication |