Combined radiation- and gentamicin-induced ototoxicity.
The aim of this project was to assess the suitability of prescribing gentamicin, a common aminoglycoside antibiotic that is known to be ototoxic, to patients undergoing radiotherapy for head and neck cancers. In clinical settings, there are cases of such patients taking gentamicin for infections. Th...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2012
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| Online Access: | http://hdl.handle.net/10356/50651 |
| _version_ | 1826129109679341568 |
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| author | Chung, Min Ru. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Chung, Min Ru. |
| author_sort | Chung, Min Ru. |
| collection | NTU |
| description | The aim of this project was to assess the suitability of prescribing gentamicin, a common aminoglycoside antibiotic that is known to be ototoxic, to patients undergoing radiotherapy for head and neck cancers. In clinical settings, there are cases of such patients taking gentamicin for infections. The gamma radiation used in radiotherapy is ototoxic and would by itself lead to sensori-neural hearing loss (SNHL) for some patients. The cell line used is the OC-k3 cell line derived from murine organ of Corti. Cell proliferation and viability assays were performed on cells treated with radiation and gentamicin for up to 72 hours post-treatment to determine proportion of cell death and extent of proliferation after treatment. Western blotting was done to elucidate the apoptotic pathways involved in apoptotic cell death induced by treatment with gentamicin and gamma radiation. The results clearly showed that OC-k3 cell death increased in a dose-dependent fashion with increasing amounts of gentamicin or radiation treatment alone. Synergistic ototoxic effects were observed when the cells were exposed to both gentamicin and radiation treatment. Western blotting results suggest that p53, PARP, and caspases of the mitochondrion-mediated pathway may play important roles in apoptotic cell death induced by gentamicin and radiation treatment. |
| first_indexed | 2024-10-01T07:35:20Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/50651 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:35:20Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/506512023-02-28T18:03:04Z Combined radiation- and gentamicin-induced ototoxicity. Chung, Min Ru. School of Biological Sciences Singapore General Hospital Low Wong Kein DRNTU::Science::Biological sciences::Cytology The aim of this project was to assess the suitability of prescribing gentamicin, a common aminoglycoside antibiotic that is known to be ototoxic, to patients undergoing radiotherapy for head and neck cancers. In clinical settings, there are cases of such patients taking gentamicin for infections. The gamma radiation used in radiotherapy is ototoxic and would by itself lead to sensori-neural hearing loss (SNHL) for some patients. The cell line used is the OC-k3 cell line derived from murine organ of Corti. Cell proliferation and viability assays were performed on cells treated with radiation and gentamicin for up to 72 hours post-treatment to determine proportion of cell death and extent of proliferation after treatment. Western blotting was done to elucidate the apoptotic pathways involved in apoptotic cell death induced by treatment with gentamicin and gamma radiation. The results clearly showed that OC-k3 cell death increased in a dose-dependent fashion with increasing amounts of gentamicin or radiation treatment alone. Synergistic ototoxic effects were observed when the cells were exposed to both gentamicin and radiation treatment. Western blotting results suggest that p53, PARP, and caspases of the mitochondrion-mediated pathway may play important roles in apoptotic cell death induced by gentamicin and radiation treatment. Bachelor of Science in Biomedical Sciences 2012-08-27T06:24:39Z 2012-08-27T06:24:39Z 2012 2012 Final Year Project (FYP) http://hdl.handle.net/10356/50651 en Nanyang Technological University 30 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Cytology Chung, Min Ru. Combined radiation- and gentamicin-induced ototoxicity. |
| title | Combined radiation- and gentamicin-induced ototoxicity. |
| title_full | Combined radiation- and gentamicin-induced ototoxicity. |
| title_fullStr | Combined radiation- and gentamicin-induced ototoxicity. |
| title_full_unstemmed | Combined radiation- and gentamicin-induced ototoxicity. |
| title_short | Combined radiation- and gentamicin-induced ototoxicity. |
| title_sort | combined radiation and gentamicin induced ototoxicity |
| topic | DRNTU::Science::Biological sciences::Cytology |
| url | http://hdl.handle.net/10356/50651 |
| work_keys_str_mv | AT chungminru combinedradiationandgentamicininducedototoxicity |