Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge
A proteolytic thermophilic bacterial strain, designated as strain SF03, was isolated from sewage sludge in Singapore. Strain SF03 is strictly aerobic, rod-shaped, Gram- positive, catalase positive, oxidase positive, and forms endospores. Strain SF03 grew at temperatures ranging from 35 to 65 OC, pH...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Research Report |
| Published: |
2008
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| Online Access: | http://hdl.handle.net/10356/5176 |
| _version_ | 1826111096116740096 |
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| author | Tay, Joo Hwa Wang, Jing Yuan Tay, Stephen Tiong Lee Stabnikova Olena Chen, Xiaoge |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Tay, Joo Hwa Wang, Jing Yuan Tay, Stephen Tiong Lee Stabnikova Olena Chen, Xiaoge |
| author_sort | Tay, Joo Hwa |
| collection | NTU |
| description | A proteolytic thermophilic bacterial strain, designated as strain SF03, was isolated from sewage sludge in Singapore. Strain SF03 is strictly aerobic, rod-shaped, Gram- positive, catalase positive, oxidase positive, and forms endospores. Strain SF03 grew at temperatures ranging from 35 to 65 OC, pH ranging from 6.0 to 9.0 and salinities ranging from 0 to 2.5%. Phylogenetic analyses revealed that strain SF03 was most similar to Saccharococcus themophilus, Geobacillus caldoxylosilyticus and Geobacillus thermoglucosidasius, with the 16s rRNA gene sequence identity of 97.6%, 97.5% and 97.2%, respectively. Based on taxonomic and 16s rRNA analysis data, strain SF03 was named Geobacillus caldoproteolyticus sp. nov. Production of extracellular protease from strain SF03 was observed in a basal peptone medium and the peptone medium supplemented with different carbon and nitrogen sources. Different levels of protease production were found with different carbon and nitrogen sources. |
| first_indexed | 2024-10-01T02:45:01Z |
| format | Research Report |
| id | ntu-10356/5176 |
| institution | Nanyang Technological University |
| last_indexed | 2024-10-01T02:45:01Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | ntu-10356/51762023-03-03T16:44:00Z Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge Tay, Joo Hwa Wang, Jing Yuan Tay, Stephen Tiong Lee Stabnikova Olena Chen, Xiaoge School of Civil and Environmental Engineering DRNTU::Engineering::Environmental engineering::Water treatment A proteolytic thermophilic bacterial strain, designated as strain SF03, was isolated from sewage sludge in Singapore. Strain SF03 is strictly aerobic, rod-shaped, Gram- positive, catalase positive, oxidase positive, and forms endospores. Strain SF03 grew at temperatures ranging from 35 to 65 OC, pH ranging from 6.0 to 9.0 and salinities ranging from 0 to 2.5%. Phylogenetic analyses revealed that strain SF03 was most similar to Saccharococcus themophilus, Geobacillus caldoxylosilyticus and Geobacillus thermoglucosidasius, with the 16s rRNA gene sequence identity of 97.6%, 97.5% and 97.2%, respectively. Based on taxonomic and 16s rRNA analysis data, strain SF03 was named Geobacillus caldoproteolyticus sp. nov. Production of extracellular protease from strain SF03 was observed in a basal peptone medium and the peptone medium supplemented with different carbon and nitrogen sources. Different levels of protease production were found with different carbon and nitrogen sources. 2008-09-17T10:31:14Z 2008-09-17T10:31:14Z 2004 2004 Research Report http://hdl.handle.net/10356/5176 Nanyang Technological University application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering::Water treatment Tay, Joo Hwa Wang, Jing Yuan Tay, Stephen Tiong Lee Stabnikova Olena Chen, Xiaoge Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge |
| title | Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge |
| title_full | Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge |
| title_fullStr | Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge |
| title_full_unstemmed | Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge |
| title_short | Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge |
| title_sort | isolation and characterization of thermoactive extracellular protease producing geobacillus caldoproteolyticus sp nov from sewage sludge |
| topic | DRNTU::Engineering::Environmental engineering::Water treatment |
| url | http://hdl.handle.net/10356/5176 |
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