Functional studies on the severe acute respiratory syndrome coronavirus envelope protein.
The envelope protein of severe acute respiratory syndrome coronavirus is a small membrane protein, with poorly defined roles in viral infection and life cycle. Recent studies have indicated its multiple functions. Beyond the conventional recognized role in virion assembly and budding, E protein also...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2013
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| Online Access: | http://hdl.handle.net/10356/52296 |
| _version_ | 1826124555581652992 |
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| author | Zhou, Tianyu. |
| author2 | Jaume Torres |
| author_facet | Jaume Torres Zhou, Tianyu. |
| author_sort | Zhou, Tianyu. |
| collection | NTU |
| description | The envelope protein of severe acute respiratory syndrome coronavirus is a small membrane protein, with poorly defined roles in viral infection and life cycle. Recent studies have indicated its multiple functions. Beyond the conventional recognized role in virion assembly and budding, E protein also has ion channel activity, and may be involved in host stress response or host-protein interactions. However, how these
functions are related to infectivity still remains debated. In this study we propose a new function of E protein, which is to induce membrane fusion, based on the observation of structural similarities with identified viral fusion peptides. We also examined the ion transport activity based on fluorescence indicator to validate the feasibility of liposome-based high-throughput screening assay for E protein inhibitor discovery. The fusion activity was unfortunately not observed, while the screening
assay based on ion transport had been validated, with several potential hits identified. |
| first_indexed | 2024-10-01T06:22:39Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/52296 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:22:39Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/522962023-02-28T18:05:33Z Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. Zhou, Tianyu. Jaume Torres School of Biological Sciences DRNTU::Science::Biological sciences::Biophysics The envelope protein of severe acute respiratory syndrome coronavirus is a small membrane protein, with poorly defined roles in viral infection and life cycle. Recent studies have indicated its multiple functions. Beyond the conventional recognized role in virion assembly and budding, E protein also has ion channel activity, and may be involved in host stress response or host-protein interactions. However, how these functions are related to infectivity still remains debated. In this study we propose a new function of E protein, which is to induce membrane fusion, based on the observation of structural similarities with identified viral fusion peptides. We also examined the ion transport activity based on fluorescence indicator to validate the feasibility of liposome-based high-throughput screening assay for E protein inhibitor discovery. The fusion activity was unfortunately not observed, while the screening assay based on ion transport had been validated, with several potential hits identified. Bachelor of Science in Biological Sciences 2013-05-06T01:04:30Z 2013-05-06T01:04:30Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/52296 en Nanyang Technological University 30 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Biophysics Zhou, Tianyu. Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. |
| title | Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. |
| title_full | Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. |
| title_fullStr | Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. |
| title_full_unstemmed | Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. |
| title_short | Functional studies on the severe acute respiratory syndrome coronavirus envelope protein. |
| title_sort | functional studies on the severe acute respiratory syndrome coronavirus envelope protein |
| topic | DRNTU::Science::Biological sciences::Biophysics |
| url | http://hdl.handle.net/10356/52296 |
| work_keys_str_mv | AT zhoutianyu functionalstudiesonthesevereacuterespiratorysyndromecoronavirusenvelopeprotein |