Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation.
Embryo implantation is the most critical stage in mammalian pregnancy. A key factor governing this process is the cytokine, Leukemia Inhibitory Factor (LIF). In mice, LIF acts on the uterine luminal epithelium (LE) converting it from a non-responsive epithelium to an embryo-responsive one. The LE ex...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2013
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| Online Access: | http://hdl.handle.net/10356/52334 |
| _version_ | 1811696695698784256 |
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| author | Yee, Li Xuan. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Yee, Li Xuan. |
| author_sort | Yee, Li Xuan. |
| collection | NTU |
| description | Embryo implantation is the most critical stage in mammalian pregnancy. A key factor governing this process is the cytokine, Leukemia Inhibitory Factor (LIF). In mice, LIF acts on the uterine luminal epithelium (LE) converting it from a non-responsive epithelium to an embryo-responsive one. The LE expresses the LIF receptor (LIFR) complex and upon stimulation by LIF, it predominantly activates the JAK/STAT pathway. However the precise sequence of events involved in such activation is unknown. We therefore employed immunofluorescence and Western blot analysis to study the sequential events involved in the activation of this pathway. Our results indicate that LIF binds to the LIFR-gp130 receptor complex, thereby phosphorylating the STAT3 transcription factor and inducing its nuclear translocation within an hour in the LE.
Apart from this pathway, LIF also activates the Wnt/β-catenin signaling both in the LE and sub-epithelial stroma (SES). In this study, we identified LEF1 and TCF4 as the interacting partner with β-catenin in the SES and LE respectively within 3 hours of LIF treatment. The robustness of the response of the LE to LIF reveals the molecular complexity of how an epithelium responds to an inductive signal and provides important leads to understand this critical process in depth. |
| first_indexed | 2024-10-01T07:43:27Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/52334 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:43:27Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/523342023-02-28T18:02:57Z Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. Yee, Li Xuan. School of Biological Sciences A*STAR Institute of Medical Biology Gracy Xavier Rosario DRNTU::Science Embryo implantation is the most critical stage in mammalian pregnancy. A key factor governing this process is the cytokine, Leukemia Inhibitory Factor (LIF). In mice, LIF acts on the uterine luminal epithelium (LE) converting it from a non-responsive epithelium to an embryo-responsive one. The LE expresses the LIF receptor (LIFR) complex and upon stimulation by LIF, it predominantly activates the JAK/STAT pathway. However the precise sequence of events involved in such activation is unknown. We therefore employed immunofluorescence and Western blot analysis to study the sequential events involved in the activation of this pathway. Our results indicate that LIF binds to the LIFR-gp130 receptor complex, thereby phosphorylating the STAT3 transcription factor and inducing its nuclear translocation within an hour in the LE. Apart from this pathway, LIF also activates the Wnt/β-catenin signaling both in the LE and sub-epithelial stroma (SES). In this study, we identified LEF1 and TCF4 as the interacting partner with β-catenin in the SES and LE respectively within 3 hours of LIF treatment. The robustness of the response of the LE to LIF reveals the molecular complexity of how an epithelium responds to an inductive signal and provides important leads to understand this critical process in depth. Bachelor of Science in Biological Sciences 2013-05-06T03:25:14Z 2013-05-06T03:25:14Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/52334 en Nanyang Technological University 43 p. application/pdf |
| spellingShingle | DRNTU::Science Yee, Li Xuan. Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. |
| title | Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. |
| title_full | Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. |
| title_fullStr | Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. |
| title_full_unstemmed | Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. |
| title_short | Leukemia inhibitory factor (LIF) regulates JAK/STAT and canonical Wnt/β-catenin signaling pathways during murine embryo implantation. |
| title_sort | leukemia inhibitory factor lif regulates jak stat and canonical wnt β catenin signaling pathways during murine embryo implantation |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/52334 |
| work_keys_str_mv | AT yeelixuan leukemiainhibitoryfactorlifregulatesjakstatandcanonicalwntbcateninsignalingpathwaysduringmurineembryoimplantation |