Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice.
Predecessor experiments have shown that microglia and myeloid cells are depleted in cd45-dtr homozygous mice but we wanted to test if cd11b-dtr/cd45-dtr heterozygous mice are able to deplete these cells just as efficiently. We generated transgenic cd11b-dtr/cd45-dtr heterozygous mice and administere...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2013
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| Online Access: | http://hdl.handle.net/10356/52456 |
| _version_ | 1826128715756601344 |
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| author | Tan, Kah Sheng. |
| author2 | Klaus Erik Karjalainen |
| author_facet | Klaus Erik Karjalainen Tan, Kah Sheng. |
| author_sort | Tan, Kah Sheng. |
| collection | NTU |
| description | Predecessor experiments have shown that microglia and myeloid cells are depleted in cd45-dtr homozygous mice but we wanted to test if cd11b-dtr/cd45-dtr heterozygous mice are able to deplete these cells just as efficiently. We generated transgenic cd11b-dtr/cd45-dtr heterozygous mice and administered different concentrations of diphtheria toxin to analyse the depletion profile of both types of mice. After which the spleen and bone marrow were analysed for myeloid cell populations via fluorescence-activated cell sorting (FACS) and cell counting under a light-field microscope. The brains were analysed for microglia via FACS. A blood analysis was carried out using a haematology analyser machine. It was found that cd11b-dtr/cd45-dtr heterozygous mice are able to deplete microglial cells (in the brain) and myeloid cells (in the spleen and bone marrow, and blood) but the depletion was not as efficient as that observed in the cd45-dtr homozygous mice. |
| first_indexed | 2024-10-01T07:29:29Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/52456 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:29:29Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/524562023-02-28T18:04:40Z Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. Tan, Kah Sheng. Klaus Erik Karjalainen School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Immunology Predecessor experiments have shown that microglia and myeloid cells are depleted in cd45-dtr homozygous mice but we wanted to test if cd11b-dtr/cd45-dtr heterozygous mice are able to deplete these cells just as efficiently. We generated transgenic cd11b-dtr/cd45-dtr heterozygous mice and administered different concentrations of diphtheria toxin to analyse the depletion profile of both types of mice. After which the spleen and bone marrow were analysed for myeloid cell populations via fluorescence-activated cell sorting (FACS) and cell counting under a light-field microscope. The brains were analysed for microglia via FACS. A blood analysis was carried out using a haematology analyser machine. It was found that cd11b-dtr/cd45-dtr heterozygous mice are able to deplete microglial cells (in the brain) and myeloid cells (in the spleen and bone marrow, and blood) but the depletion was not as efficient as that observed in the cd45-dtr homozygous mice. Bachelor of Science in Biological Sciences 2013-05-09T03:32:18Z 2013-05-09T03:32:18Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/52456 en Nanyang Technological University 34 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Immunology Tan, Kah Sheng. Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. |
| title | Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. |
| title_full | Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. |
| title_fullStr | Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. |
| title_full_unstemmed | Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. |
| title_short | Analysing the depletion of microglia and myeloid cells in the cd11b-dtr/cd45-dtr mice. |
| title_sort | analysing the depletion of microglia and myeloid cells in the cd11b dtr cd45 dtr mice |
| topic | DRNTU::Science::Biological sciences::Microbiology::Immunology |
| url | http://hdl.handle.net/10356/52456 |
| work_keys_str_mv | AT tankahsheng analysingthedepletionofmicrogliaandmyeloidcellsinthecd11bdtrcd45dtrmice |