Analysis of zebrafish mutants from Ac/Ds transposon screen.
Zebrafish is a suitable model organism to research due to certain unique traits which includes; embryo transparency, short generation time, advancement in sequenced genome and high homology percentage. These traits allow zebrafish to be easily studied using mutagenesis. The aim of this project is to...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/52703 |
| _version_ | 1811676800745472000 |
|---|---|
| author | Koh, Eirene Rae Jia Xuan. |
| author2 | Karuna Sampath |
| author_facet | Karuna Sampath Koh, Eirene Rae Jia Xuan. |
| author_sort | Koh, Eirene Rae Jia Xuan. |
| collection | NTU |
| description | Zebrafish is a suitable model organism to research due to certain unique traits which includes; embryo transparency, short generation time, advancement in sequenced genome and high homology percentage. These traits allow zebrafish to be easily studied using mutagenesis. The aim of this project is to screen out homozygous zebrafish mutants generated by Ac/Ds transposon system. The Ds cassette and Ac transposase were co-injected into founder embryos and these fish were raised to adulthood and out-crossed with wild type zebrafish. F1 embryos with florescence reporter gene expression were raised to adulthood and sent for sequencing. Subsequently, zebrafish from each line that were positive for Ds cassettes insertion were in-crossed to generate homozygous mutants. Genomic DNA from progeny fish were analysed by PCR and Southern blot. Homozygous mutants are then characterised for observable phenotypes in early embryonic development. In this project, 45 zebrafish lines have been screened. Carriers of 2 mutant genes, ptenb and SCR, had been found and characterised. Ptenb homozygous mutants were generated for a particular fish line and mutant edema phenotype was observed. However, no conclusive link between the edema phenotype and the ptenb mutant was found. There is a need to screen for more homozygous mutants for characterisation. |
| first_indexed | 2024-10-01T02:27:14Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/52703 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:27:14Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/527032023-02-28T18:04:43Z Analysis of zebrafish mutants from Ac/Ds transposon screen. Koh, Eirene Rae Jia Xuan. Karuna Sampath School of Biological Sciences Temasek Life Sciences Laboratory DRNTU::Science Zebrafish is a suitable model organism to research due to certain unique traits which includes; embryo transparency, short generation time, advancement in sequenced genome and high homology percentage. These traits allow zebrafish to be easily studied using mutagenesis. The aim of this project is to screen out homozygous zebrafish mutants generated by Ac/Ds transposon system. The Ds cassette and Ac transposase were co-injected into founder embryos and these fish were raised to adulthood and out-crossed with wild type zebrafish. F1 embryos with florescence reporter gene expression were raised to adulthood and sent for sequencing. Subsequently, zebrafish from each line that were positive for Ds cassettes insertion were in-crossed to generate homozygous mutants. Genomic DNA from progeny fish were analysed by PCR and Southern blot. Homozygous mutants are then characterised for observable phenotypes in early embryonic development. In this project, 45 zebrafish lines have been screened. Carriers of 2 mutant genes, ptenb and SCR, had been found and characterised. Ptenb homozygous mutants were generated for a particular fish line and mutant edema phenotype was observed. However, no conclusive link between the edema phenotype and the ptenb mutant was found. There is a need to screen for more homozygous mutants for characterisation. Bachelor of Science in Biological Sciences 2013-05-22T07:26:39Z 2013-05-22T07:26:39Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/52703 en Nanyang Technological University 31 p. application/pdf |
| spellingShingle | DRNTU::Science Koh, Eirene Rae Jia Xuan. Analysis of zebrafish mutants from Ac/Ds transposon screen. |
| title | Analysis of zebrafish mutants from Ac/Ds transposon screen. |
| title_full | Analysis of zebrafish mutants from Ac/Ds transposon screen. |
| title_fullStr | Analysis of zebrafish mutants from Ac/Ds transposon screen. |
| title_full_unstemmed | Analysis of zebrafish mutants from Ac/Ds transposon screen. |
| title_short | Analysis of zebrafish mutants from Ac/Ds transposon screen. |
| title_sort | analysis of zebrafish mutants from ac ds transposon screen |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/52703 |
| work_keys_str_mv | AT koheireneraejiaxuan analysisofzebrafishmutantsfromacdstransposonscreen |