Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®.
Pseudomonas spp. biofilms typically grow in aqueous environments of high shear and flow rate. Despite the conditions, the biofilms are able to retain signaling molecules that are involved in QS and biofilm development through EPS. While it is unknown how these molecules bind, it has been proposed th...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2013
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| Online Access: | http://hdl.handle.net/10356/52908 |
| _version_ | 1811692663191109632 |
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| author | Gea, Chong Jin. |
| author2 | Susana Geifman Shochat |
| author_facet | Susana Geifman Shochat Gea, Chong Jin. |
| author_sort | Gea, Chong Jin. |
| collection | NTU |
| description | Pseudomonas spp. biofilms typically grow in aqueous environments of high shear and flow rate. Despite the conditions, the biofilms are able to retain signaling molecules that are involved in QS and biofilm development through EPS. While it is unknown how these molecules bind, it has been proposed that these signaling molecules are retained by an amyloid fibril, FapC, found in the EPS. The Biacore™ T200, EnSpire® and LigandTracer® were employed to study and characterize the interactions of these signaling molecules and also of potential inhibitors of biofilm formation with FapC. The results from the T200 indicated that the signaling molecules bound to FapC with a relative strong affinity but with possible effect from his-tag, and revealed potentially strong binders from a list of small molecules selected from a previous photofluorescence screening performed by Dr Kim Junyoung from the Chemical Bioimaging Laboratory, NUS. Optimization of the immobilization of FapC in the EnSpire® suggested conditions similar to those in the T200, while more optimization in the LigandTracer® is needed due to difficulty in immobilizing FapC. To conclude, signaling molecules and other small molecules were found to bind to FapC using Biacore T200™. |
| first_indexed | 2024-10-01T06:39:22Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/52908 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:39:22Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/529082023-02-28T18:03:01Z Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. Gea, Chong Jin. Susana Geifman Shochat School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Bacteria DRNTU::Science::Biological sciences::Biophysics Pseudomonas spp. biofilms typically grow in aqueous environments of high shear and flow rate. Despite the conditions, the biofilms are able to retain signaling molecules that are involved in QS and biofilm development through EPS. While it is unknown how these molecules bind, it has been proposed that these signaling molecules are retained by an amyloid fibril, FapC, found in the EPS. The Biacore™ T200, EnSpire® and LigandTracer® were employed to study and characterize the interactions of these signaling molecules and also of potential inhibitors of biofilm formation with FapC. The results from the T200 indicated that the signaling molecules bound to FapC with a relative strong affinity but with possible effect from his-tag, and revealed potentially strong binders from a list of small molecules selected from a previous photofluorescence screening performed by Dr Kim Junyoung from the Chemical Bioimaging Laboratory, NUS. Optimization of the immobilization of FapC in the EnSpire® suggested conditions similar to those in the T200, while more optimization in the LigandTracer® is needed due to difficulty in immobilizing FapC. To conclude, signaling molecules and other small molecules were found to bind to FapC using Biacore T200™. Bachelor of Science in Biological Sciences 2013-05-29T02:55:05Z 2013-05-29T02:55:05Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/52908 en Nanyang Technological University 39 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Bacteria DRNTU::Science::Biological sciences::Biophysics Gea, Chong Jin. Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. |
| title | Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. |
| title_full | Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. |
| title_fullStr | Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. |
| title_full_unstemmed | Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. |
| title_short | Study and characterization of the interactions of the recently discovered functional amyloid fibril, FapC with signaling molecules and potential inhibitors with three biosensors : Biacore™ T200, EnSpire® and LigandTracer®. |
| title_sort | study and characterization of the interactions of the recently discovered functional amyloid fibril fapc with signaling molecules and potential inhibitors with three biosensors biacore™ t200 enspire r and ligandtracer r |
| topic | DRNTU::Science::Biological sciences::Microbiology::Bacteria DRNTU::Science::Biological sciences::Biophysics |
| url | http://hdl.handle.net/10356/52908 |
| work_keys_str_mv | AT geachongjin studyandcharacterizationoftheinteractionsoftherecentlydiscoveredfunctionalamyloidfibrilfapcwithsignalingmoleculesandpotentialinhibitorswiththreebiosensorsbiacoret200enspireandligandtracer |