Cytotoxicity of carriers for the delivery of drugs to cancer cells
Although the concept of drug delivery system is old, its application has widened recently. An appreciable progress has been implemented in the treatment of lethal diseases such as cancer, targeting the delivery of drugs as one important aspect. For drug delivery, the carriers to load and unload the...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/53662 |
| _version_ | 1811678796078645248 |
|---|---|
| author | Divya, Venkataraman |
| author2 | Bjoern Holger Neu |
| author_facet | Bjoern Holger Neu Divya, Venkataraman |
| author_sort | Divya, Venkataraman |
| collection | NTU |
| description | Although the concept of drug delivery system is old, its application has widened recently. An appreciable progress has been implemented in the treatment of lethal diseases such as cancer, targeting the delivery of drugs as one important aspect. For drug delivery, the carriers to load and unload the drugs should be biocompatible and biodegradable in nature for in vivo applications. To fabricate suitable carriers for targeted and controlled release of drugs, the Layer by Layer(LbL) technique has invoked a great interest in the field of biomedical engineering. LbL method ensures consecutive adsorption of alternatively charged polyelectrolyte pairs on the porous colloidal templates. The choice of polyelectrolyte pairs and the core for the drug delivery application has remained an issue for several years. The main purpose of this project is the study of in vitro cytotoxicity of the carriers to the MCF-7 human breast cancer cell line. The carriers used in this study are polyelectrolyte coated on the porous colloidal templates, the CaCO3 micro particles and hydroxyapatite nanoparticles. The surface characterization study proved the particle stability by zeta potential measurements. Additionally, qualitative study of the internalization of the carrier particles proved successful uptake by the model 3T3 mouse fibroblast cells and the target MCF-7 human breast cancer cells. Also, the extent of cytotoxic response elicited by the target cells on treatment with varying concentration of the carrier particles was studied using the MTT(3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay. This proved that the concentration of the carriers used were not significantly cytotoxic to the target cells. Thus to conclude, the fabricated carriers are highly biocompatible and suitable for various in vivo applications in the drug delivery system. |
| first_indexed | 2024-10-01T02:58:57Z |
| format | Thesis |
| id | ntu-10356/53662 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:58:57Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/536622023-03-11T17:09:48Z Cytotoxicity of carriers for the delivery of drugs to cancer cells Divya, Venkataraman Bjoern Holger Neu School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering Although the concept of drug delivery system is old, its application has widened recently. An appreciable progress has been implemented in the treatment of lethal diseases such as cancer, targeting the delivery of drugs as one important aspect. For drug delivery, the carriers to load and unload the drugs should be biocompatible and biodegradable in nature for in vivo applications. To fabricate suitable carriers for targeted and controlled release of drugs, the Layer by Layer(LbL) technique has invoked a great interest in the field of biomedical engineering. LbL method ensures consecutive adsorption of alternatively charged polyelectrolyte pairs on the porous colloidal templates. The choice of polyelectrolyte pairs and the core for the drug delivery application has remained an issue for several years. The main purpose of this project is the study of in vitro cytotoxicity of the carriers to the MCF-7 human breast cancer cell line. The carriers used in this study are polyelectrolyte coated on the porous colloidal templates, the CaCO3 micro particles and hydroxyapatite nanoparticles. The surface characterization study proved the particle stability by zeta potential measurements. Additionally, qualitative study of the internalization of the carrier particles proved successful uptake by the model 3T3 mouse fibroblast cells and the target MCF-7 human breast cancer cells. Also, the extent of cytotoxic response elicited by the target cells on treatment with varying concentration of the carrier particles was studied using the MTT(3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay. This proved that the concentration of the carriers used were not significantly cytotoxic to the target cells. Thus to conclude, the fabricated carriers are highly biocompatible and suitable for various in vivo applications in the drug delivery system. Master of Science (Biomedical Engineering) 2013-06-06T08:28:37Z 2013-06-06T08:28:37Z 2011 2011 Thesis http://hdl.handle.net/10356/53662 en 89 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Mechanical engineering Divya, Venkataraman Cytotoxicity of carriers for the delivery of drugs to cancer cells |
| title | Cytotoxicity of carriers for the delivery of drugs to cancer cells |
| title_full | Cytotoxicity of carriers for the delivery of drugs to cancer cells |
| title_fullStr | Cytotoxicity of carriers for the delivery of drugs to cancer cells |
| title_full_unstemmed | Cytotoxicity of carriers for the delivery of drugs to cancer cells |
| title_short | Cytotoxicity of carriers for the delivery of drugs to cancer cells |
| title_sort | cytotoxicity of carriers for the delivery of drugs to cancer cells |
| topic | DRNTU::Engineering::Mechanical engineering |
| url | http://hdl.handle.net/10356/53662 |
| work_keys_str_mv | AT divyavenkataraman cytotoxicityofcarriersforthedeliveryofdrugstocancercells |