Metabolic engineering of bacteria cells through construction of plasmid.
Artemisinin is one drug that is critically needed in the current market to treat the disease malaria. This type of drug is mostly being produced in the Artemisia annua plant. However, in many countries, the production of artemisinin by Artemisia annua plant has proven to be insufficient and many res...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/54564 |
| _version_ | 1826118395418902528 |
|---|---|
| author | Leow, Shi Jie. |
| author2 | Song Hao |
| author_facet | Song Hao Leow, Shi Jie. |
| author_sort | Leow, Shi Jie. |
| collection | NTU |
| description | Artemisinin is one drug that is critically needed in the current market to treat the disease malaria. This type of drug is mostly being produced in the Artemisia annua plant. However, in many countries, the production of artemisinin by Artemisia annua plant has proven to be insufficient and many researchers have resort to looking in various directions hoping to find new ways of enhancing the overall amorphadiene production.
Metabolic engineering of bacteria such as the Escherichia Coli and the Saccharomyces Cerevisiae bacteria to produce respective drugs are commonly used. One of the metabolic engineering techniques studied in this report is the creating of new plasmids through combination of the important genetic information and injecting them in to these groups of bacteria cells to enhance production level.
The steps taken to conduct the experiment will be mentioned in the later part of the report. In addition, the various types of laboratory equipment and their respective roles will also be revealed. |
| first_indexed | 2024-10-01T04:42:41Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/54564 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:42:41Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/545642023-03-03T15:35:19Z Metabolic engineering of bacteria cells through construction of plasmid. Leow, Shi Jie. Song Hao School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Artemisinin is one drug that is critically needed in the current market to treat the disease malaria. This type of drug is mostly being produced in the Artemisia annua plant. However, in many countries, the production of artemisinin by Artemisia annua plant has proven to be insufficient and many researchers have resort to looking in various directions hoping to find new ways of enhancing the overall amorphadiene production. Metabolic engineering of bacteria such as the Escherichia Coli and the Saccharomyces Cerevisiae bacteria to produce respective drugs are commonly used. One of the metabolic engineering techniques studied in this report is the creating of new plasmids through combination of the important genetic information and injecting them in to these groups of bacteria cells to enhance production level. The steps taken to conduct the experiment will be mentioned in the later part of the report. In addition, the various types of laboratory equipment and their respective roles will also be revealed. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2013-06-24T03:10:37Z 2013-06-24T03:10:37Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/54564 en Nanyang Technological University 57 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Bioengineering Leow, Shi Jie. Metabolic engineering of bacteria cells through construction of plasmid. |
| title | Metabolic engineering of bacteria cells through construction of plasmid. |
| title_full | Metabolic engineering of bacteria cells through construction of plasmid. |
| title_fullStr | Metabolic engineering of bacteria cells through construction of plasmid. |
| title_full_unstemmed | Metabolic engineering of bacteria cells through construction of plasmid. |
| title_short | Metabolic engineering of bacteria cells through construction of plasmid. |
| title_sort | metabolic engineering of bacteria cells through construction of plasmid |
| topic | DRNTU::Engineering::Bioengineering |
| url | http://hdl.handle.net/10356/54564 |
| work_keys_str_mv | AT leowshijie metabolicengineeringofbacteriacellsthroughconstructionofplasmid |