Engineering G-quadruplex DNA : a combined computational and experimental study
G-quadruplex nucleic acids have received a great deal of research attention for their biological functions, therapeutic potential, and roles in nanotechnology. The research focus of this thesis is dedicated to the engineering of G-quadruplex DNA structures. We approach this problem through a combina...
| 主要作者: | |
|---|---|
| 其他作者: | |
| 格式: | Thesis |
| 语言: | English |
| 出版: |
2014
|
| 主题: | |
| 在线阅读: | http://hdl.handle.net/10356/55380 |
| _version_ | 1826130677915975680 |
|---|---|
| author | Lech, Christopher Jacques |
| author2 | Phan Anh Tuan |
| author_facet | Phan Anh Tuan Lech, Christopher Jacques |
| author_sort | Lech, Christopher Jacques |
| collection | NTU |
| description | G-quadruplex nucleic acids have received a great deal of research attention for their biological functions, therapeutic potential, and roles in nanotechnology. The research focus of this thesis is dedicated to the engineering of G-quadruplex DNA structures. We approach this problem through a combination of experimental and computational approaches. We examine some of the fundamental interactions that govern G-quadruplex structure and their high order assemblies. In addition, we investigate the effects of G-quadruplex geometry on electronic conductance of stacked G-tetrads. A large part of our research efforts deal with the development and application of alternative nucleic acid chemistries within G-quadruplex structures. These include chemical modifications to the guanine base and to the sugar moiety of the backbone. We demonstrate how these chemical tools may be used to enhance some beneficial properties G-quadruplexes and to rationally design stable and functionalizable G-quadruplex nanoparticles for applications in nanotechnology and therapeutics |
| first_indexed | 2024-10-01T08:00:09Z |
| format | Thesis |
| id | ntu-10356/55380 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T08:00:09Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/553802023-02-28T23:56:41Z Engineering G-quadruplex DNA : a combined computational and experimental study Lech, Christopher Jacques Phan Anh Tuan School of Physical and Mathematical Sciences DRNTU::Science G-quadruplex nucleic acids have received a great deal of research attention for their biological functions, therapeutic potential, and roles in nanotechnology. The research focus of this thesis is dedicated to the engineering of G-quadruplex DNA structures. We approach this problem through a combination of experimental and computational approaches. We examine some of the fundamental interactions that govern G-quadruplex structure and their high order assemblies. In addition, we investigate the effects of G-quadruplex geometry on electronic conductance of stacked G-tetrads. A large part of our research efforts deal with the development and application of alternative nucleic acid chemistries within G-quadruplex structures. These include chemical modifications to the guanine base and to the sugar moiety of the backbone. We demonstrate how these chemical tools may be used to enhance some beneficial properties G-quadruplexes and to rationally design stable and functionalizable G-quadruplex nanoparticles for applications in nanotechnology and therapeutics Doctor of Philosophy (SPMS) 2014-02-25T11:47:38Z 2014-02-25T11:47:38Z 2013 2013 Thesis http://hdl.handle.net/10356/55380 10.32657/10356/55380 en 184 p. application/pdf |
| spellingShingle | DRNTU::Science Lech, Christopher Jacques Engineering G-quadruplex DNA : a combined computational and experimental study |
| title | Engineering G-quadruplex DNA : a combined computational and experimental study |
| title_full | Engineering G-quadruplex DNA : a combined computational and experimental study |
| title_fullStr | Engineering G-quadruplex DNA : a combined computational and experimental study |
| title_full_unstemmed | Engineering G-quadruplex DNA : a combined computational and experimental study |
| title_short | Engineering G-quadruplex DNA : a combined computational and experimental study |
| title_sort | engineering g quadruplex dna a combined computational and experimental study |
| topic | DRNTU::Science |
| url | http://hdl.handle.net/10356/55380 |
| work_keys_str_mv | AT lechchristopherjacques engineeringgquadruplexdnaacombinedcomputationalandexperimentalstudy |